API 5000 LC/MS/MS System Package

API 5000 LC/MS/MS 系统套件

• 批准号: 7219842
• 负责人: Peter C Dedon
• 金额: $ 42.43万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7219842
• 关键词: Affect; Antineoplastic Agents; Area; Atmospheric Pressure; Biological; Biological Markers; Biomedical Engineering; Chemicals; Cities; Communities; DNA; Devices; Drug Design; Environmental Health; Faculty; Fostering; Foxes; Funding; Goals; Human; Infectious Agent; Inflammation; Lipids; Liver; Measures; Mechanical Stress; Methods; Proteins; Proteomics; Public Health; Range; Research; Research Project Grants; Scientist; Site; Specificity; Stress; System; Tissues; United States National Institutes of Health; base; bench to bedside; drug metabolism; human disease; human tissue; ion source; ionization; mass spectrometer; microbial alkaline proteinase inhibitor; response; systems research

DESCRIPTION (provided by applicant): We are requesting funds for the purchase a new API 5000 tandem quadrupole LC- MS/MS system, with turboionspray and atmospheric-pressure chemical ionization ion sources, manufactured by Applied Biosystems of Foster City, CA. The device will be used by the eight participating faculty, as well as other scientists in the MIT community, to quantify and characterize biological and synthetic molecules in studies affecting at least eighteen NIH-funded research projects. Our current single triple quadrupole mass spectrometer (API 3000) in the Center for Environmental Health Sciences (CEHS) is becoming overwhelmed by sharply increasing demand for LC-MS/MS analyses requiring greater sensitivity, mainly in the area of biomarker quantification. The co-applicants all have demonstrated needs for the API 5000 system for research projects funded by the NIH: Peter Dedon, John Essigmann, James Fox, Alan Grodzinsky, Leona Samson, David Schauer, Steven Tannenbaum, and John Wishnok. The projects that would benefit from the API 5000 range widely from proteomic analyses of cellular responses to infectious agents and mechanical stresses; anticancer drug design; drug metabolism in bioengineered liver tissue; to DNA, RNA, protein and lipid biomarkers of oxidative and nitrosative stress arising at sites of inflammation in humans. In all cases, the goal is to take advantage of the sensitivity and chemical specificity of the API 5000 triple quadrupole mass spectrometer system to rigorously quantify synthetic and biological molecules at the relatively low levels that occur in diseased and healthy human tissues, with sensitivities in the range of attomoles to femtomoles. No other system meets these demands. The public health relevance of the studies that would be supported by the API 5000 lies in their goals to develop sensitive methods to quantify molecules involved in both the therapy and the pathophysiological basis for human diseases. Sensitive measuring devices such as the API 5000 are crucial to the objective of moving NIH-sponsored research into humans - from bench to bedside.

描述(由申请人提供)：我们正在申请资金，用于购买新的API 5000串联四极LC-MS/MS系统，具有涡轮喷雾和常压化学电离离子源，由加利福尼亚州福斯特市的应用生物系统公司制造。该设备将由八名参与的教员以及麻省理工学院社区的其他科学家使用，在影响至少18个NIH资助的研究项目的研究中量化和表征生物和合成分子。我们目前位于环境健康科学中心(CEHS)的单三重四极杆质谱仪(API 3000)由于对LC-MS/MS分析的需求急剧增加而变得不堪重负，这些分析需要更高的灵敏度，主要是在生物标志物定量领域。共同申请者都证明了NIH资助的研究项目对API 5000系统的需求：Peter Dedon、John Essigmann、James Fox、Alan Grodzinsky、Leona Samson、David Schauer、Steven Tannenbaum和John Wishnok。将受益于API 5000的项目范围广泛，包括对细胞对感染剂和机械压力的反应的蛋白质组分析；抗癌药物设计；生物工程肝组织中的药物新陈代谢；以及人类炎症部位氧化和亚硝化应激的DNA、RNA、蛋白质和脂肪生物标记物。在所有情况下，我们的目标都是利用API 5000三重四极杆质谱仪系统的灵敏度和化学特异度，严格定量疾病和健康人体组织中相对较低水平的合成和生物分子，其灵敏度在attomoles到fetomoles的范围内。没有其他系统能满足这些要求。API 5000支持的这些研究的公共卫生相关性在于他们的目标是开发灵敏的方法来量化人类疾病的治疗和病理生理学基础中涉及的分子。像API 5000这样的灵敏测量设备对于将NIH赞助的研究从工作台转移到床边的目标至关重要。