API 5000 LC/MS/MS System Package

API 5000 LC/MS/MS 系统套件

• 批准号: 7219842
• 负责人: Peter C Dedon
• 金额: $ 42.43万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7219842
• 关键词: Affect; Antineoplastic Agents; Area; Atmospheric Pressure; Biological; Biological Markers; Biomedical Engineering; Chemicals; Cities; Communities; DNA; Devices; Drug Design; Environmental Health; Faculty; Fostering; Foxes; Funding; Goals; Human; Infectious Agent; Inflammation; Lipids; Liver; Measures; Mechanical Stress; Methods; Proteins; Proteomics; Public Health; Range; Research; Research Project Grants; Scientist; Site; Specificity; Stress; System; Tissues; United States National Institutes of Health; base; bench to bedside; drug metabolism; human disease; human tissue; ion source; ionization; mass spectrometer; microbial alkaline proteinase inhibitor; response; systems research

DESCRIPTION (provided by applicant): We are requesting funds for the purchase a new API 5000 tandem quadrupole LC- MS/MS system, with turboionspray and atmospheric-pressure chemical ionization ion sources, manufactured by Applied Biosystems of Foster City, CA. The device will be used by the eight participating faculty, as well as other scientists in the MIT community, to quantify and characterize biological and synthetic molecules in studies affecting at least eighteen NIH-funded research projects. Our current single triple quadrupole mass spectrometer (API 3000) in the Center for Environmental Health Sciences (CEHS) is becoming overwhelmed by sharply increasing demand for LC-MS/MS analyses requiring greater sensitivity, mainly in the area of biomarker quantification. The co-applicants all have demonstrated needs for the API 5000 system for research projects funded by the NIH: Peter Dedon, John Essigmann, James Fox, Alan Grodzinsky, Leona Samson, David Schauer, Steven Tannenbaum, and John Wishnok. The projects that would benefit from the API 5000 range widely from proteomic analyses of cellular responses to infectious agents and mechanical stresses; anticancer drug design; drug metabolism in bioengineered liver tissue; to DNA, RNA, protein and lipid biomarkers of oxidative and nitrosative stress arising at sites of inflammation in humans. In all cases, the goal is to take advantage of the sensitivity and chemical specificity of the API 5000 triple quadrupole mass spectrometer system to rigorously quantify synthetic and biological molecules at the relatively low levels that occur in diseased and healthy human tissues, with sensitivities in the range of attomoles to femtomoles. No other system meets these demands. The public health relevance of the studies that would be supported by the API 5000 lies in their goals to develop sensitive methods to quantify molecules involved in both the therapy and the pathophysiological basis for human diseases. Sensitive measuring devices such as the API 5000 are crucial to the objective of moving NIH-sponsored research into humans - from bench to bedside.

描述（由申请人提供）：我们正在申请资金购买一个新的API 5000串联四极杆LC- MS/MS系统，具有涡轮喷雾和大气压化学电离离子源，由加利福尼亚州福斯特市的应用生物系统公司制造。该设备将由8名参与的教师以及麻省理工学院社区的其他科学家使用，在影响至少18个nih资助的研究项目的研究中量化和表征生物和合成分子。我们目前在环境健康科学中心（CEHS）的单三重四极杆质谱仪（API 3000）正变得不堪重负，因为对LC-MS/MS分析的需求急剧增加，需要更高的灵敏度，主要是在生物标志物定量领域。共同申请人都证明了由NIH资助的研究项目对API 5000系统的需求：Peter Dedon， John Essigmann, James Fox, Alan Grodzinsky, Leona Samson, David Schauer， Steven Tannenbaum和John Wishnok。受益于API 5000的项目范围广泛，包括细胞对感染因子和机械应力反应的蛋白质组学分析；抗癌药物设计；生物工程肝组织药物代谢研究到人类炎症部位产生的氧化应激和亚硝化应激的DNA、RNA、蛋白质和脂质生物标志物。在所有情况下，目标是利用API 5000三重四极杆质谱仪系统的灵敏度和化学特异性，严格量化在患病和健康人体组织中相对较低水平的合成和生物分子，灵敏度在原子摩尔到飞摩尔范围内。没有其他系统能满足这些要求。API 5000支持的研究与公共卫生的相关性在于它们的目标是开发敏感的方法来量化涉及人类疾病治疗和病理生理基础的分子。灵敏的测量设备，如API 5000，对于将nih资助的研究从实验室转移到临床至关重要。