API 5000 LC/MS/MS System Package

描述（由申请人提供）：我们正在申请资金，用于购买一台新的API 5000串联四极杆LC- MS/MS系统，配备涡轮离子喷雾和大气压化学电离离子源，由Applied Biosystems of Foster City，CA生产。该设备将由八名参与教师以及麻省理工学院社区的其他科学家使用，以量化和表征影响至少18个NIH资助研究项目的研究中的生物和合成分子。我们目前在环境健康科学中心（CEHS）使用的单三重四极杆质谱仪（API 3000），由于对LC-MS/MS分析的需求急剧增加，主要是在生物标志物定量方面，需要更高的灵敏度，因此变得不堪重负。共同申请人都证明了NIH资助的研究项目对API 5000系统的需求：Peter Dedon、John Essigmann、James Fox、Alan Grodzinsky、Leona Samson、大卫Schauer、Steven Tannenbaum和John Wishnok。受益于API 5000的项目范围广泛，从对感染因子和机械应力的细胞反应的蛋白质组学分析;抗癌药物设计;生物工程肝组织中的药物代谢;到人类炎症部位产生的氧化和亚硝化应激的DNA、RNA、蛋白质和脂质生物标志物。在所有情况下，目标是利用API 5000三重四极杆质谱仪系统的灵敏度和化学特异性，以相对低的水平严格定量患病和健康人体组织中存在的合成和生物分子，灵敏度在阿托摩尔至飞摩尔范围内。没有其他系统能满足这些要求。API 5000支持的研究的公共卫生相关性在于其目标是开发敏感的方法来量化参与人类疾病治疗和病理生理基础的分子。灵敏的测量设备，如API 5000是至关重要的目标，移动NIH赞助的研究到人类-从实验室到床边。

DESCRIPTION (provided by applicant): We are requesting funds for the purchase a new API 5000 tandem quadrupole LC- MS/MS system, with turboionspray and atmospheric-pressure chemical ionization ion sources, manufactured by Applied Biosystems of Foster City, CA. The device will be used by the eight participating faculty, as well as other scientists in the MIT community, to quantify and characterize biological and synthetic molecules in studies affecting at least eighteen NIH-funded research projects. Our current single triple quadrupole mass spectrometer (API 3000) in the Center for Environmental Health Sciences (CEHS) is becoming overwhelmed by sharply increasing demand for LC-MS/MS analyses requiring greater sensitivity, mainly in the area of biomarker quantification. The co-applicants all have demonstrated needs for the API 5000 system for research projects funded by the NIH: Peter Dedon, John Essigmann, James Fox, Alan Grodzinsky, Leona Samson, David Schauer, Steven Tannenbaum, and John Wishnok. The projects that would benefit from the API 5000 range widely from proteomic analyses of cellular responses to infectious agents and mechanical stresses; anticancer drug design; drug metabolism in bioengineered liver tissue; to DNA, RNA, protein and lipid biomarkers of oxidative and nitrosative stress arising at sites of inflammation in humans. In all cases, the goal is to take advantage of the sensitivity and chemical specificity of the API 5000 triple quadrupole mass spectrometer system to rigorously quantify synthetic and biological molecules at the relatively low levels that occur in diseased and healthy human tissues, with sensitivities in the range of attomoles to femtomoles. No other system meets these demands. The public health relevance of the studies that would be supported by the API 5000 lies in their goals to develop sensitive methods to quantify molecules involved in both the therapy and the pathophysiological basis for human diseases. Sensitive measuring devices such as the API 5000 are crucial to the objective of moving NIH-sponsored research into humans - from bench to bedside.