API 5000 LC/MS/MS System Package

描述（由申请人提供）：我们正在申请资金购买新的 API 5000 串联四极杆 LC-MS/MS 系统，该系统配有涡轮离子喷雾和大气压化学电离离子源，由加利福尼亚州福斯特城的 Applied Biosystems 制造。该设备将由 8 名参与的教师以及麻省理工学院的其他科学家使用，以量化和表征研究中的生物和合成分子，这些研究影响至少 18 个 NIH 资助的研究项目。我们环境健康科学中心 (CEHS) 目前的单三重四极杆质谱仪 (API 3000) 因对 LC-MS/MS 分析的需求急剧增加而不堪重负，这些分析需要更高的灵敏度，主要是在生物标志物定量领域。共同申请人均证明了 NIH 资助的研究项目对 API 5000 系统的需求：Peter Dedon、John Essigmann、James Fox、Alan Grodzinsky、Leona Samson、David Schauer、Steven Tannenbaum 和 John Wishnok。受益于 API 5000 的项目范围广泛，包括细胞对感染因子和机械应力反应的蛋白质组学分析；抗癌药物设计；生物工程肝组织中的药物代谢；人类炎症部位产生的氧化和亚硝化应激的 DNA、RNA、蛋白质和脂质生物标志物。在所有情况下，目标都是利用 API 5000 三重四极杆质谱仪系统的灵敏度和化学特异性，严格定量患病和健康人体组织中相对较低水平的合成分子和生物分子，灵敏度在阿托摩尔到飞摩尔范围内。没有其他系统可以满足这些要求。 API 5000 支持的研究与公共卫生的相关性在于其目标是开发灵敏的方法来量化参与人类疾病的治疗和病理生理学基础的分子。 API 5000 等敏感测量设备对于实现 NIH 资助的人体研究从实验室到临床的目标至关重要。

DESCRIPTION (provided by applicant): We are requesting funds for the purchase a new API 5000 tandem quadrupole LC- MS/MS system, with turboionspray and atmospheric-pressure chemical ionization ion sources, manufactured by Applied Biosystems of Foster City, CA. The device will be used by the eight participating faculty, as well as other scientists in the MIT community, to quantify and characterize biological and synthetic molecules in studies affecting at least eighteen NIH-funded research projects. Our current single triple quadrupole mass spectrometer (API 3000) in the Center for Environmental Health Sciences (CEHS) is becoming overwhelmed by sharply increasing demand for LC-MS/MS analyses requiring greater sensitivity, mainly in the area of biomarker quantification. The co-applicants all have demonstrated needs for the API 5000 system for research projects funded by the NIH: Peter Dedon, John Essigmann, James Fox, Alan Grodzinsky, Leona Samson, David Schauer, Steven Tannenbaum, and John Wishnok. The projects that would benefit from the API 5000 range widely from proteomic analyses of cellular responses to infectious agents and mechanical stresses; anticancer drug design; drug metabolism in bioengineered liver tissue; to DNA, RNA, protein and lipid biomarkers of oxidative and nitrosative stress arising at sites of inflammation in humans. In all cases, the goal is to take advantage of the sensitivity and chemical specificity of the API 5000 triple quadrupole mass spectrometer system to rigorously quantify synthetic and biological molecules at the relatively low levels that occur in diseased and healthy human tissues, with sensitivities in the range of attomoles to femtomoles. No other system meets these demands. The public health relevance of the studies that would be supported by the API 5000 lies in their goals to develop sensitive methods to quantify molecules involved in both the therapy and the pathophysiological basis for human diseases. Sensitive measuring devices such as the API 5000 are crucial to the objective of moving NIH-sponsored research into humans - from bench to bedside.