Upgrade of a Campus NMR Facility for Biological Solids

校园生物固体核磁共振设施升级

• 批准号: 7215520
• 负责人: Leonard J Mueller
• 金额: $ 27.75万
• 依托单位: UNIVERSITY OF CALIFORNIA RIVERSIDE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7215520
• 关键词: 1 year old; Agonist; Analytical Chemistry; Binding; Biochemistry; Biological; Biophysics; Cations; Chemicals; Chemistry; Development; Drug Delivery Systems; Equipment; Goals; Insulin; Intuition; Liquid substance; Magnetic Resonance; Methods; Molecular; Nuclear Magnetic Resonance; Oral; Organic Chemistry; Phase; Range; Science; Site; Solid; Structure; System; Therapeutic Agents; Tryptophan Synthase; Universities; Urination; design; instrumentation; molecular imaging; next generation; novel; solid state

DESCRIPTION (provided by applicant): We propose to upgrade an existing 600 MHz Bruker AVANCE liquid-state nuclear magnetic resonance spectrometer to perform NMR on biological solids. The current machine (1-year old) and the additionally requested equipment will become part of the University's Analytical Chemistry Instrumentation Facility (ACIF). Solid-state NMR has become an essential method of characterization for a wide range of medicinal systems in chemistry, biochemistry, materials science and biophysics. Its large impact within the University is witnessed by the diversity of projects put forth in this proposal. In particular, Michael Dunn and Len Mueller will study covalent intermediates bound to the ¿-site of tryptophan synthase, Michael Pirrung will characterize solid-phase synthetic oral insulin mimics, Katie DeFea and Len Mueller will determine the solid-state structure of PAR-2 agonists, and Chris Reed will investigate reactive cations in inorganic and organic chemistry. These problems all share a common need for solid-state characterization. Although well equipped for liquid-state NMR, there are currently no University-wide facilities capable of solid-state NMR at UCR. As such, solid-state NMR equipment will fill a noticeable void in the types of NMR characterization that can be performed at UC Riverside. Relevance: The relationship between structure and biological activity drives the development of therapeutic agents. Here we propose to add a significant molecular imaging capability through solid-state magnetic resonance. The goal is to determine the molecular level details that govern the interaction between drug and target molecules and build our chemical intuition for designing novel, next-generation treatments.

描述(申请人提供)：我们建议对现有的600 MHz Bruker Avance液态核磁共振波谱仪进行升级，以对生物固体进行核磁共振。目前的机器(使用1年)和额外要求的设备将成为大学分析化学仪器设施(ACIF)的一部分。固体核磁共振已经成为化学、生物化学、材料科学和生物物理学中广泛的医学体系表征的基本方法。它在大学内的巨大影响体现在这项提案中提出的各种项目上。特别是，Michael Dunn和Len Mueller将研究绑定到色氨酸合成酶？位的共价中间体，Michael Pirrung将表征固相合成口服胰岛素模拟物，Katie Defea和Len Mueller将确定PAR-2激动剂的固态结构，Chris Reed将研究无机化学和有机化学中的活性阳离子。所有这些问题都有一个共同的需求，即固态表征。尽管配备了良好的液态核磁共振设备，但目前UCR还没有大学范围内能够进行固态核磁共振的设施。因此，固态核磁共振设备将填补加州大学河滨分校可以进行的核磁共振表征类型中的一个明显空白。 相关性：结构和生物活性之间的关系推动了治疗剂的发展。在这里，我们建议通过固态磁共振增加一个重要的分子成像能力。我们的目标是确定控制药物和目标分子之间相互作用的分子水平细节，并为设计新的下一代治疗方法建立我们的化学直觉。

项目成果

10-Methyldodecanal, a Novel Attractant Pheromone Produced by Males of the South American Cerambycid Beetle Eburodacrys vittata.

10-甲基十二醛，一种由南美天牛 Eburodacrys vittata 雄性产生的新型引诱信息素。

• DOI: 10.1371/journal.pone.0160727
• 发表时间: 2016
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Silva,WelitonD;Millar,JocelynG;Hanks,LawrenceM;Bento,JoséMaurícioS
• 通讯作者: Bento,JoséMaurícioS

Novel, male-produced aggregation pheromone of the cerambycid beetle Rosalia alpina, a priority species of European conservation concern.

新型男性生产的聚集信息素，cerambycid甲虫Rosalia alpina，这是欧洲保护问题的优先物种。

• DOI: 10.1371/journal.pone.0183279
• 发表时间: 2017
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Žunič Kosi A;Zou Y;Hoskovec M;Vrezec A;Stritih N;Millar JG
• 通讯作者: Millar JG

Chelation of a proton by an aliphatic tertiary diamine.

质子与脂肪族叔二胺的螯合。

• DOI: 10.1021/ja8018725
• 发表时间: 2008
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: Yaghmaei,Sepideh;Khodagholian,Sevana;Kaiser,JMichael;Tham,FookS;Mueller,LeonardJ;Morton,ThomasHellman
• 通讯作者: Morton,ThomasHellman

31P NMR investigation of backbone dynamics in DNA binding sites.

DNA 结合位点主链动力学的 31P NMR 研究。

• DOI: 10.1021/jp711203m
• 发表时间: 2009
• 期刊: The journal of physical chemistry. B
• 作者: Tian,Ye;Kayatta,Michael;Shultis,Katharine;Gonzalez,Alejandro;Mueller,LeonardJ;Hatcher,MaryE
• 通讯作者: Hatcher,MaryE