600 MHz NMR Spectrometer and CPMAS CryoProbe

项目摘要： 我们建议购买第一个用于固态NMR的CPMAS冷冻探针， 美国，沿着专用600 MHz核磁共振光谱仪，支持生物分子研究 在加州大学滨江和周围的南加州地区。这种增加的敏感性 该工具将支持由NIH资助的变革性研究项目，包括： 异染色质形成和基因沉默，神经退行性变的结构转化 疾病相关蛋白质（淀粉样蛋白），病原体相互作用的膜蛋白效应物， 吡哆醛-5'-磷酸盐依赖性寄主、机理酶学和化学结构/动力学 酶、β-内酰胺酶的抑制和生物聚合物的力学。这些问题都有一个共同点， 在实验灵敏度的极限下进行分子表征的共同需要。尽管如此- 配备固态NMR，支持常规和动态核极化（DNP） 检测，目前在南加州或北美没有设施， 所需仪器对必须保持在室温或室温附近的样品的灵敏度 温度因此，该设备将填补核磁共振表征类型中的一个明显空白 可以在区域和国家范围内执行。拟议的冷冻探针和光谱仪将 成为加州大学滨江分校分析化学仪器的一部分 设施，为校园，当地社区和来自全国各地的外部用户提供服务。

Project Summary: We propose the acquisition of the first CPMAS cryoprobe for solid-state NMR to be housed in the United States, along with a dedicated 600 MHz NMR spectrometer to support biomolecular research at UC Riverside and the surrounding Southern California region. The increased sensitivity of this instrument will enable transformative research projects supported by NIH funding that include: heterochromatin formation and gene silencing, structural transformations of neurodegenerative disease related proteins (amyloids), membrane protein effectors of pathogen interactions with the host, mechanistic enzymology and chemical structure/dynamics of pyridoxal-5'-phosphate-dependent enzymes, inhibition of β-lactamases, and the mechanics of biopolymers. These problems all share a common need for molecular characterization at the limits of experimental sensitivity. Although well- equipped for solid-state NMR with conventional and dynamic nuclear polarization (DNP) enabled detection, there are currently no facilities within Southern California or North America with the sensitivity of the requested instrumentation for samples that must be maintained at or near room temperature. As such, this equipment will fill a noticeable void in the types of NMR characterization that can be performed both regionally and nationally. The proposed cryoprobe and spectrometer will become part of the University of California at Riverside’s Analytical Chemistry Instrumentation Facility, which serves the campus, local community, and external users from across the country.