600 MHz NMR Spectrometer and CPMAS CryoProbe

600 MHz NMR 波谱仪和 CPMAS CryoProbe

• 批准号: 10415784
• 负责人: Leonard J Mueller
• 金额: $ 199.29万
• 依托单位: UNIVERSITY OF CALIFORNIA RIVERSIDE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-16 至 2024-09-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10415784
• 关键词: Amyloid; Analytical Chemistry; Biopolymers; California; Chemical Structure; Communities; Country; Detection; Enzymatic Biochemistry; Enzyme Inhibition; Equipment; Funding; Gene Silencing; Heterochromatin; Mechanics; Membrane Proteins; Molecular; NMR Spectroscopy; Neurodegenerative Disorders; North America; Nuclear; Proteins; Pyridoxal Phosphate; Research; Research Project Grants; Sampling; Temperature; United States; United States National Institutes of Health; Universities; beta-Lactamase; instrument; instrumentation; pathogen; solid state nuclear magnetic resonance

Project Summary: We propose the acquisition of the first CPMAS cryoprobe for solid-state NMR to be housed in the United States, along with a dedicated 600 MHz NMR spectrometer to support biomolecular research at UC Riverside and the surrounding Southern California region. The increased sensitivity of this instrument will enable transformative research projects supported by NIH funding that include: heterochromatin formation and gene silencing, structural transformations of neurodegenerative disease related proteins (amyloids), membrane protein effectors of pathogen interactions with the host, mechanistic enzymology and chemical structure/dynamics of pyridoxal-5'-phosphate-dependent enzymes, inhibition of β-lactamases, and the mechanics of biopolymers. These problems all share a common need for molecular characterization at the limits of experimental sensitivity. Although well- equipped for solid-state NMR with conventional and dynamic nuclear polarization (DNP) enabled detection, there are currently no facilities within Southern California or North America with the sensitivity of the requested instrumentation for samples that must be maintained at or near room temperature. As such, this equipment will fill a noticeable void in the types of NMR characterization that can be performed both regionally and nationally. The proposed cryoprobe and spectrometer will become part of the University of California at Riverside’s Analytical Chemistry Instrumentation Facility, which serves the campus, local community, and external users from across the country.

项目概要： 我们建议购买第一个用于固态 NMR 的 CPMAS 冷冻探针，安装在 美国，配有专用的 600 MHz NMR 波谱仪来支持生物分子研究 加州大学河滨分校和周边南加州地区。这个灵敏度的提高 该仪器将支持 NIH 资助的变革性研究项目，其中包括： 异染色质形成和基因沉默，神经退行性疾病的结构转变 疾病相关蛋白（淀粉样蛋白）、病原体与细胞相互作用的膜蛋白效应子 5'-磷酸吡哆醛依赖的宿主、机械酶学和化学结构/动力学 酶、β-内酰胺酶的抑制以及生物聚合物的力学。这些问题都有一个共同点 在实验灵敏度极限下进行分子表征的普遍需要。虽然很好—— 配备固态 NMR，支持常规和动态核极化 (DNP) 检测，目前南加州或北美没有设施具有 要求仪器对必须保存在室温或接近室温的样品的灵敏度 温度。因此，该设备将填补 NMR 表征类型中的明显空白 这可以在区域和国家范围内进行。拟议的冷冻探针和光谱仪将 成为加州大学河滨分校分析化学仪器的一部分 该设施为校园、当地社区和来自全国各地的外部用户提供服务。