Computed microscopy: solving the inverse problem of optical microscopy to image deeper into biological tissue

计算机显微镜：解决光学显微镜的逆问题，以更深入地成像生物组织

• 批准号: FT140100849
• 负责人: Dr Peter Munro
• 金额: --
• 依托单位: The University of Western Australia
• 依托单位国家: 澳大利亚
• 项目类别: ARC Future Fellowships
• 财政年份: 2014
• 资助国家: 澳大利亚
• 起止时间: 2014-07-01 至 2015-03-13
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/FT140100849
• 关键词: Computed; microscopy; solving; inverse; problem

This project aims to enable 3D optical microscopy to image deeper within tissue, ultimately aiding research fields such as neurobiology. This will be achieved by a foundational approach called computed cicroscopy that combines novel numerical methods, high performance computing and optical microscopy. This project aims to develop a 3D quantitative imaging method that will provide unprecedented insight into the structure of tissue with sub-cellular detail. This information can then be used to computationally reverse light scattering within the sample, allowing fluorescence microscopy at unprecedented depths within tissue.

该项目旨在使3D光学显微镜能够在组织内部更深处成像，最终帮助神经生物学等研究领域。这将通过一种称为计算环镜的基础方法来实现，该方法结合了新颖的数值方法、高性能计算和光学显微镜。该项目旨在开发一种3D定量成像方法，该方法将提供前所未有的亚细胞细节组织结构的洞察力。然后，这些信息可以用于计算逆转样品内的光散射，允许荧光显微镜在组织内前所未有的深度。