3D Imaging of electrical activity in myocardial tissue

心肌组织电活动的 3D 成像

• 批准号: 7234014
• 负责人: Arkady M PERTSOV
• 金额: $ 56.02万
• 依托单位: UPSTATE MEDICAL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7234014
• 关键词: 3-Dimensional; Action Potentials; Address; Algorithms; Arrhythmia; Behavior; Binding; Boa; Cardiac Myocytes; Characteristics; Charge; Chemicals; Collection; Computer Simulation; Connecticut; Coupled; Cyclodextrins; Depth; Development; Devices; Dyes; Embryonic Heart; Family; Filament; Fluorescence; Fluorescent Dyes; Fluorescent Probes; Foundations; General Hospitals; Generations; Goals; Health; Heart; Image; Imaging Device; Imaging technology; Lead; Light; Maintenance; Massachusetts; Measurement; Measures; Medical; Membrane Potentials; Modeling; Myocardial; Myocardial tissue; Optical Tomography; Optics; Penetration; Pharmacology; Physics; Research; Resolution; Signal Transduction; Source; Surface; System; Tail; Technology; Testing; Three-Dimensional Image; Three-Dimensional Imaging; Tissues; Universities; Ventricular Fibrillation; Work; absorption; base; bioimaging; design; improved; medical schools; millimeter; new technology; optical imaging; prototype; reconstruction; second harmonic; size; two-photon; voltage

DESCRIPTION (provided by applicant): Understanding the mechanisms that underlie abnormalities of electrical conduction in the heart is the key to the development of effective antiarrhythmic therapies. During the last decade, significant progress has been made in imaging electrical excitation waves in the heart using voltage-sensitive fluorescent dyes. However, until recently imaging using voltage-sensitive dyes was limited primarily to the epicardial surface. The goal of the proposed study is to develop a technology that would enable optical imaging of electrical excitation throughout the myocardial wall. Specifically, this technology should image the filaments, or organizing centers of vortex-like electrical activity. These are widely believed to be responsible for the initiation and maintenance of ventricular fibrillation, and the filaments are a key to their behavior. To address the technical challenges of this novel technology we propose a coordinated project involving the research groups of Dr. A. Pertsov (PI) from the Department of Pharmacology, SUNY Upstate Medical University, who pioneered the three-dimensional imaging of vortex-like excitation in chemical excitable systems and in the heart; Dr. D. Boas at the Harvard Medical School and the Massachusetts General Hospital NMR Center, an expert in optical tomography; Dr. L. Loew at the Center for Biomedical Imaging Technology, University of Connecticut Health Center (Co-PI), a leader in the development of voltage-sensitive probes and optical imaging; and the group of Dr. D. Weitz at the Department of Physics, Harvard University (Co-PI), renowned for their expertise in optical imaging and multiple-scattering media. The specific aims of the project are: 1) to create realistic computer models for reconstructing 2D optical images from 3D distributions of the transmembrane potential in myocardial tissue (forward problem), 2) to apply diffusive optical tomography to 3D reconstruction of the actual electrical activation in the heart (inverse problem); 3) to design, synthesize and test in myocardial tissues a family of near-infrared voltage-sensitive dyes optimized for 3D imaging of electrical activation in the heart; 4) to explore two-photon fluorescence and second-harmonic generation for 3D imaging of electrical activity in cardiac myocytes and tissues at subcellular and sub-millimeter scales. Successful completion of this project will break ground for a new technology, the 3D imaging of electrical activation in the heart.

描述（由申请人提供）：了解心脏电传导异常的机制是开发有效抗心律失常疗法的关键。在过去的十年中，使用电压敏感荧光染料对心脏中的电激发波进行成像已经取得了重大进展。然而，直到最近，使用电压敏感染料的成像主要局限于心外膜表面。拟议研究的目标是开发一种技术，能够对整个心肌壁的电激发进行光学成像。具体来说，这项技术应该对细丝或涡流状电活动的组织中心进行成像。人们普遍认为这些纤维是心室颤动的引发和维持的原因，而细丝是其行为的关键。 为了解决这项新技术的技术挑战，我们提出了一个协调项目，该项目涉及纽约州立大学上州医科大学药理学系的 A. Pertsov 博士（PI）的研究小组，他开创了化学可兴奋系统和心脏中涡旋状激发的三维成像；哈佛医学院和麻省总医院核磁共振中心的 D. Boas 博士，光学断层扫描专家；康涅狄格大学健康中心生物医学成像技术中心 (Co-PI) 的 L. Loew 博士，电压敏感探针和光学成像开发领域的领导者；以及哈佛大学物理系的 D. Weitz 博士团队（Co-PI），该团队以其在光学成像和多重散射介质方面的专业知识而闻名。 该项目的具体目标是：1) 创建真实的计算机模型，用于根据心肌组织中跨膜电位的 3D 分布重建 2D 光学图像（正向问题），2) 将扩散光学断层扫描应用于心脏中实际电激活的 3D 重建（逆向问题）； 3) 在心肌组织中设计、合成和测试一系列近红外电压敏感染料，这些染料针对心脏电激活的 3D 成像进行了优化； 4) 探索双光子荧光和二次谐波产生，用于亚细胞和亚毫米尺度心肌细胞和组织电活动的 3D 成像。该项目的成功完成将为一项新技术——心脏电激活的 3D 成像——奠定基础。

项目成果

Intra-myocardial cusp waves and their manifestation in optical mapping signals.

心肌内尖波及其在光学映射信号中的表现。

• DOI: 10.1109/iembs.2006.260852
• 发表时间: 2006
• 期刊: Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference
• 作者: Bernus,Olivier;Zemlin,ChristianW;Matiukas,Arvydas;Hyatt,ChristopherJ;Pertsov,ArkadyM
• 通讯作者: Pertsov,ArkadyM

Frustrated drift of an anchored scroll-wave filament and the geodesic principle.

• DOI: 10.1103/physreve.82.036122
• 发表时间: 2010-09
• 期刊: Physical review. E, Statistical, nonlinear, and soft matter physics
• 作者: M. Wellner;C. Zemlin;A. Pertsov

Optical mapping of electrical heterogeneities in the heart during global ischemia.

• DOI: 10.1109/iembs.2009.5333176
• 发表时间: 2009
• 期刊: Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
• 作者: Matiukas A;Pertsov AM;Kothari P;Cram A;Tolkacheva EG
• 通讯作者: Tolkacheva EG

Method for the three-dimensional localization of intramyocardial excitation centers using optical imaging.

使用光学成像对心肌内激励中心进行三维定位的方法。

• DOI: 10.1117/1.2204030
• 发表时间: 2006
• 期刊: Journal of biomedical optics
• 影响因子: 3.5
• 作者: Khait,VadimD;Bernus,Olivier;Mironov,SergeyF;Pertsov,ArkadyM
• 通讯作者: Pertsov,ArkadyM

Nonlinear optical potentiometric dyes optimized for imaging with 1064-nm light.

非线性光学电位染料针对 1064 nm 光成像进行了优化。

• DOI: 10.1117/1.2772276
• 发表时间: 2007
• 期刊: Journal of biomedical optics
• 影响因子: 3.5
• 作者: Teisseyre,ThomasZ;Millard,AndrewC;Yan,Ping;Wuskell,JosephP;Wei,Mei-de;Lewis,Aaron;Loew,LeslieM
• 通讯作者: Loew,LeslieM