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Depth-resolved imaging of myocardial excitation

心肌兴奋的深度分辨成像

基本信息

批准号：
8098007
负责人：
Arkady M PERTSOV
金额：
$ 27.48万
依托单位：
UPSTATE MEDICAL UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2003
资助国家：
美国
起止时间：
2003-07-15 至 2013-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8098007
关键词：
Action Potentials Acute Advanced Development Algorithms Anatomy Anti-Arrhythmia Agents Arrhythmia Arteries Blood Cardiac Characteristics Cicatrix Clinical Complex Computer Simulation Coronary Coronary artery Data Dyes Electrodes Electrophysiology (science)Family suidae Fingers Fluorescent Dyes Gadolinium Goals Health Hybrids Image Imaging technology Individual Infarction Ischemia Island Lasers Lateral Left Link Location Magnetic Resonance Imaging Maps Methods Modeling Myocardial Myocardium Optics Pattern Perfusion Preparation Prevention therapy Radiofrequency Interstitial Ablation Reporting Resolution Role Scanning Simulate Site Source Staging Staining method Stains Surface System Techniques Technology Testing Three-Dimensional Imaging Time Ventricular artery occlusion charge coupled device camera clinical application clinically relevant feeding gadolinium oxide hyperkalemia improved minimally invasive new technology novel optical imaging reconstruction research study success sudden cardiac death voltage

项目摘要

DESCRIPTION (provided by applicant): The development of advanced technologies for electrophysiological mapping of cardiac propagation has been a defining factor in recent successes both in basic understanding of arrhythmia mechanisms and clinical applications such as mapping-guided radiofrequency ablation. However, one of the major challenges both in basic and clinical cardiac electrophysiology remains the mapping of abnormally conducting arrhythmogenic regions hidden inside the myocardial wall. The ultimate goal of this proposal is to develop a minimally invasive 3D tomographic optical mapping technique utilizing novel near-infrared (NIR) voltage-sensitive dyes, which will significantly enhance one's ability to study electrical excitation inside the myocardial wall. During the previous period of support, we have conducted extensive modeling and experimental studies that indicate the feasibility of our method. The next step will be to implement, validate, and assess the potential of the new technique for 3D mapping of normal myocardium and physiologically relevant arrhythmogenic ischemic substrates. The Specific Aims of this proposal are: 1) Build a fast optical tomographic system for the time-resolved 3D imaging of intra-mural electrical waves in isolated coronary-perfused ventricular wall preparations. 2) Perform the 3D reconstructions of intramural electrical excitation wave in Tyrode-perfused and blood-perfused pig ventricular wall preparations. 3) Test the accuracy of the reconstructions under conditions simulating non- uniform staining patterns characteristic of arrhythmogenic substrates (ischemic border, infarction scar). 4) Utilize the new techniques to determine the role of 3D geometry of perfusion territories in arrhythmogenesis during acute regional ischemia. We will test the hypothesis that the anatomy of the perfusion territory of a given coronary artery defines the geometry of the ischemic region and is a major factor that determines the pattern of electrical propagation emerging after occlusion of that artery. Successful completion of the proposed Specific Aims will produce, validate, and test both under normal and clinically relevant conditions a fast new tomographic method for the 3D optical imaging of intra-myocardial excitation. It will also provide new mechanistic information regarding the links between the anatomy of perfusion territories and ischemia-related conduction abnormalities. PUBLIC HEALTH RELEVANCE: The goal of the proposed studies is to develop an optical imaging technology for detecting sources of arrhythmia hidden deep inside the myocardial wall. Such sources emerge during the blockage of arteries feeding cardiac muscle and often cause sudden cardiac death. The new technology will help to understand the mechanisms of initiation of such sources, which is the key to developing effective anti-arrhythmic therapies and prevention of sudden cardiac death.

描述（由申请人提供）：心脏传播电生理标测的先进技术的发展是近年来在心律失常机制的基本理解和标测引导射频消融等临床应用方面取得成功的决定性因素。然而，在基础和临床心脏电生理学中的主要挑战之一仍然是隐藏在心肌壁内的异常传导致心律失常区域的标测。这项建议的最终目标是开发一种微创3D断层光学映射技术，利用新的近红外（NIR）电压敏感染料，这将显着提高人们的能力，研究心肌壁内的电激励。在前一段时间的支持，我们已经进行了广泛的建模和实验研究，表明我们的方法的可行性。下一步将是实施、验证和评估新技术用于正常心肌和生理学相关致心肌缺血基质的3D标测的潜力。本研究的具体目标是：1）建立一个快速的光学断层成像系统，用于孤立的冠状动脉灌注心室壁标本中壁内电波的时间分辨三维成像。2)在灌注Tyrode和血液的猪心室壁标本上进行壁内电兴奋波的三维重建。3)在模拟致瘤基质（缺血边界、梗死瘢痕）的非均匀染色模式特征的条件下，测试重建的准确性。4)利用新技术确定急性局部缺血期间灌注区域的3D几何形状在血管生成中的作用。我们将检验这样一个假设，即给定冠状动脉灌注区域的解剖结构定义了缺血区域的几何形状，并且是决定该动脉闭塞后出现的电传播模式的主要因素。成功完成拟议的特定目标将在正常和临床相关条件下产生、验证和测试一种用于心肌内激发3D光学成像的快速新断层摄影方法。它还将提供新的机制信息之间的联系，解剖灌注领土和缺血相关的传导异常。公共卫生相关性：这项研究的目的是开发一种光学成像技术，用于检测隐藏在心肌壁深处的心律失常源。这些来源出现在供应心肌的动脉阻塞期间，通常会导致心脏性猝死。这项新技术将有助于了解这些来源的启动机制，这是开发有效的抗心律失常治疗和预防心脏性猝死的关键。