Peptide hydrogels: A 3D in vitro model for liver toxicity.

肽水凝胶：肝毒性的 3D 体外模型。

• 批准号: 2905682
• 金额: --
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2905682
• 关键词: Peptide; hydrogels; 3D; vitro; model

Liver diseases are a significant medical burden, due to the lack of donors. There is a heavy global reliance on animal testing with a wide variety of animals used, and a major cause of cessation of drug development is secondary liver toxicity. The most common toxicity associated with drug withdrawals in the US and Europe are hepatic at 21%. This project exploits an engineered 3D culture system that maintains hepatocyte specific functions for liver toxicity testing using self-assembled peptide hydrogels that mimic the hepatic microenvironment. Optimal gels will be characterised for material physical and chemical properties, cell behaviour (using biochemical assays, immunocytochemistry/imaging and gene and protein expression), to determine the optimal gel system that can support the appropriate cells in long term culture, that can allow the cells to respond to toxins. This may then prove to be an appropriate in vitro 3D culture platform for liver toxicity testing.

由于缺乏捐赠者，肝病是一项重大的医疗负担。全球严重依赖动物试验，使用的动物种类繁多，药物开发停止的一个主要原因是继发性肝毒性。在美国和欧洲，与停药相关的最常见毒性是肝脏，占21%。该项目利用一种工程化的3D培养系统，利用模拟肝脏微环境的自组装肽水凝胶，维持肝细胞特异性功能进行肝毒性测试。最佳凝胶将被表征为材料的物理和化学性质，细胞行为（使用生化分析，免疫细胞化学/成像和基因和蛋白质表达），以确定最佳的凝胶系统，可以支持适当的细胞在长期培养，可以让细胞对毒素作出反应。这可能会被证明是一个合适的体外3D培养平台肝毒性测试。