Biomolecular Analysis Core D

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The Biomolecular Analysis Core increases efficiency and cost effectiveness by performing mass spectrometric and certain other analyses for CNRU investigators. The Core provides state-of-the-art mass spectrometry to determine structures and to quantify nutrients and related substances in biological specimens. The Core provides centralized, standardized analytical procedures that permit study of molecular mechanisms for nutrient-related physiologic and pathophysiologic events. Specific objectives of the Biomolecular Analysis Core are: To provide training to students and fellows in principles of mass spectrometry (MS) and use of MS systems, e.g., gas chromatography (GC)/MS, isotope ratio (IR)/MS, electrospray ionization (ESI)/tandem MS, and matrix assisted laser desorption ionization (MALDI)/time of flight/ (TOF)/MS in analysis of nutrition-related biomolecules. To develop new MS methods for structural identification and quantitation of molecules involved in nutrition-related physiologic and pathophysiologic events. To perform service-related MS analyses, e.g. quantitation of target analytes and obtaining spectra for structural identification. To assist CNRU investigators in developing MS assays. To provide consultation in interpreting MS data. To develop and disseminate new approaches in biomedical MS applicable to nutrition research. To provide and maintain functional MS systems for use in nutrition research. To perform collaborative nutrition research studies involving Biomolecular Analysis Core staff. To reduce nutrition research-related costs by providing centralized MS services and consultation at a fraction of the cost for commercial MS services or of maintaining instruments in individual investigators' laboratories.