Imaging and Microscopy Core

成像和显微镜核心

• 批准号: 7315888
• 负责人: DALE B HALES
• 金额: $ 11.73万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7315888
• 关键词: Antibodies; Antigens; Apoptosis; Arts; Avidin; Bibliography; Biological Assay; Biological Markers; Biotin; Color; Complex; Computer Workstations; Computer software; Condition; Confocal Microscopy; Consultations; Cultured Cells; Data Analyses; Databases; Development; Dissection; Documentation; Dry Ice; Electron Microscopy; Electrons; Endometrial; Equipment; Evaluation; Fluorescence; Freezing; Frozen Sections; Genes; Goals; Grant; Histology; Human; Human Resources; Illinois; Image; Image Analysis; Imagery; Immunofluorescence Immunologic; Immunohistochemistry; In Situ Hybridization; In Situ Nick-End Labeling; Internet; Laboratories; Laser Microscopy; Lasers; Link; Localized; Location; Medicine; Messenger RNA; Methodology; Methods; Microscope; Microscopy; Microtome - medical device; PCNA gene; Papio; Paraffin Embedding; Plastics; Post Technic; Postdoctoral Fellow; Preparation; Price; Printing; Process; Protocols documentation; Publications; Research; Research Personnel; Resources; Retrieval; S-Phase Fraction; Sampling; Savings; Schedule; Science; Series; Services; Slide; Spottings; Staining method; Stains; Standards of Weights and Measures; Students; System; Testing; Time; Tissue Microarray; Tissue Procurements; Tissue Sample; Tissues; Training; United States National Institutes of Health; Universities; base; college; computerized data processing; cost; cryostat; digital; digital imaging; endometriosis; human tissue; image processing; immunocytochemistry; investigator training; laser capture microdissection; research study; sample fixation; tissue fixing; tissue preparation; tissue processing; tissue/cell preparation

The Core moved into the College of Medicine Research Building into new laboratory space with consolidation and centralization of all equipment and services into one location. A website for the Imaging and Microscopy Core was implemented containing: a database of antibodies available for immunohistochemistry with application specific examples; downloadable histology protocols; interactive web-form to submit inquiries to Core personnel; and a detailed list of services and price schedules, plus a bibliography of publications supported the Core. Services provided by the Core: In addition to standard histology support, the Core provided: ¿ Training and consultation for Center personnel * Consultation of the various fixation methods for tissues was available to those who required assistance and trouble shooting for antigen retrieval methods * Antibody testing for determination of optimal conditions * Immunofluorescence of cultured cells * Tissue preparation for electron microscopy * Tissue and cell preparation for confocal microscopy * Antibody purification for use in immunohistochemistry * TUNEL staining in fixed sections for apoptosis studies Dual color TUNEL and PCNA or Ki-67 staining for apoptosis/proliferation index * In situ hybridization for localizing mRNA in tissue sections (fluorescent and colorometric visualization) Image analysis. The Core provided services to assist investigators with image acquisition and documentation of results using our image analysis workstation. Using the Nikon Eclipse E400 series microscope, single images are captured, processed and printed with a fully functional computer workstation. The Image-Pro Plus software and NIH Image J are used for quantitative morphometric analyses. Serve as a training center. The Core has trained investigators, pre- and post-doctoral students as well as technicians of U54 investigators in the art and science of immunohistochemistry, in situ hybridization, image analysis and data processing.

核心团队搬入医学院研究大楼并整合到新的实验室空间 将所有设备和服务集中到一个位置。 实施了成像和显微镜核心网站，其中包含： 抗体数据库 可用于免疫组织化学并附有具体应用示例；可下载的组织学协议； 交互式网络表格，用于向核心人员提交查询；以及详细的服务清单和价格表， 加上支持核心的出版物参考书目。 核心提供的服务： 除了标准组织学支持外，核心还提供： ¿ 中心人员培训和咨询 * 有需要者可咨询各种组织固定方法 抗原修复方法的协助和故障排除 * 抗体测试以确定最佳条件 * 培养细胞的免疫荧光 * 电子显微镜的组织准备 * 共聚焦显微镜的组织和细胞制备 * 用于免疫组织化学的抗体纯化 * 固定切片中的 TUNEL 染色用于细胞凋亡研究 双色 TUNEL 和 PCNA 或 Ki-67 染色检测细胞凋亡/增殖指数 * 原位杂交用于定位组织切片中的 mRNA（荧光和比色可视化） 图像分析。核心提供服务以协助调查人员进行图像采集和记录 使用我们的图像分析工作站得出的结果。使用 Nikon Eclipse E400 系列显微镜，单 图像由功能齐全的计算机工作站捕获、处理和打印。 Image-Pro Plus 软件和 NIH Image J 用于定量形态分析。 充当培训中心。该核心培训了研究人员、博士前和博士后学生以及 U54 免疫组织化学、原位杂交、图像艺术和科学研究人员的技术人员 分析和数据处理。