Center for Women's Health and Reproduction

加州大学生殖研究专业合作中心计划的目标 伊利诺伊州将在生殖科学方面进行创新的基础和临床研究。提出了四个项目。 3项目I和n侧重于子宫内膜异位症的病因学和病理生理学，而项目I和项目IV则侧重于 在啮齿动物模型中蜕膜化和植入的相关问题。在细胞周期调控方面获得的见解， 然而，这些啮齿动物模型中的细胞凋亡和金属蛋白酶将直接适用于理解 人类生殖的心理学和病理生物学。项目一“浣熊子宫内膜异位症”--将检验这一假设 子宫内膜异位症的发展分为两个不同的阶段。产生的数据将决定卵巢是否 如果局部类固醇的生物合成能维持这种疾病，那么类固醇对于疾病的确立是必要的。该项目还将 建立子宫内膜异位症与子宫容受性异常之间的直接联系。项目II“#年的激素行动” 子宫内膜异位症“-将检验子宫内膜异位症组织中孕酮抵抗干扰子宫内膜异位症诱导的假说 CEY雌二醇(E2)代谢酶17/β-羟基脱氢酶(HSD)2型。一个主要目标是确定 核受体表达异常导致孕酮抵抗和17P-HSD-2缺失导致 在子宫内膜异位症中，组织中E2水平的升高是细胞命运改变的原因。PI计划“细胞信号与基因 蜕膜发育的调控“将侧重于蜕膜衍生蛋白的功能作用。使用转基因 NICE模型，细胞周期抑制物、激活素A和催乳素在发育和维持中的作用和调节 )是否对妊娠期间的蜕膜进行评估。项目IV：EMMPRIN调节血管内金属蛋白酶 子宫内膜“验证了一种假说，即子宫分泌的金属蛋白酶诱导物(EMMPRIN) 子宫内膜上皮细胞调节子宫内膜中金属蛋白酶的产生。这些研究将集中在 基质金属蛋白酶在着床中的重要性，并确定EMMPRIN的表达是否是产生基质金属蛋白酶所必需的 核仁组织。这些项目将得到一个行政核心和两个研究核心的支持：a)成像和 显微镜和B)组织获取和细胞培养。这些研究核心将以开放获取模式运作 MD支持NIH批准的其他7项拨款，这些拨款的研究目标将有所贡献，但不会违反 他是U54申请者。伊利诺伊大学U54中心将在强大的互动性和 大学！在一个在生殖研究方面有丰富历史的机构里。

The objective of the Specialized Cooperative Centers Program in Reproductive Research (SCCPRR) at the University of llinois is to conduct innovative basic and clinical research in the reproductive sciences. Four projects are proposed. 3rojects I and n focus on the etiology and pathophysiology of endometriosis while Projects HI and IV address important questions relating to decidualization and implantation in rodent models. Insights gained on the regulation of cell cycle, ipoptosis and metalloproteinases in these rodent models, however, will be directly applicable to understanding the jhysiology and pathobiology of human reproduction. Project I "Endometriosis in the Baboon" - will test the hypothesis hat the development of endometriosis occurs in two distinct phases. The data generated will determine if ovarian steroids are necessary to first establish the disease and if local steroid biosynthesis will maintain it. This project will also establish a direct link between endometriosis and aberrations in uterine receptivity. Project II "Hormone Action in indometriosis" - will test the hypothesis that progesterone resistance in endometriotic tissue disrupts the induction of the cey estradiol (E2) metabolizing enzyme 17/?-hydroxydehydrogenase (HSD) type 2. A major goal is to determine whether iberrations in nuclear receptor expression causing progesterone resistance and the absence of 17P-HSD-2 giving rise to increased tissue E2 levels are responsible for altered cell fate in endometriosis. Project PI "Cell Signaling and Gene Regulation in Decidual Development" will focus on the functional role of decidual-derived proteins. Using transgenic nice models, the role and regulation of cell cycle inhibitors, activin A and prolactin in the development and maintenance )f the decidua during pregnancy will be evaluated. Project IV "EMMPRIN Regulates Metalloproteinases in the Endometrium" tests the hypothesis that an inducer of metalloproteinases (EMMPRIN) that is secreted by uterine epithelial cells regulates metalloproteinase (MMP) production in the endometrium. These studies will focus on the importance of MMPs in implantation and determine if EMMPRIN expression is required for MMP production by ;ndometriotic tissue. These projects will be supported by an administrative core and two research cores: A) Imaging and Microscopy and B) Tissue Procurement and Cell Culture. These research cores will operate in an open access formula md support 7 other NIH approved grants whose research objectives will contribute but will not infringe upon the goals of he U54 application. The U54 Center at the University of Illinois will be established within a strong interactive and collegia! environment and in an institution that has a rich history in reproductive research.