Characterization of Cell-Polymer Surface Interactions

细胞-聚合物表面相互作用的表征

基本信息

批准号：
7268852
负责人：
CHARLENE RINCON
金额：
$ 4.25万
依托单位：
GEORGIA INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-08-01 至 2008-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7268852
关键词：
Adhesions Adhesives Affect Atomic Force Microscopy Behavior Biocompatible Materials Biological Assay Bone Regeneration Caliber Cell Proliferation Cell physiology Cell surface Cell-Matrix Junction Cells Characteristics Chemical Structure Chemicals Chemistry Chloroform Complex Computer software Condition Coupled Coupling Cues Cultured Cells Cyclohexane Cyclohexanes Defect Development Devices Diagnostic Dust Electric Conductivity Electric Stimulation Electrodes Electrons Engineering Fellowship Film Free Energy Glass Goals Growth Head Healed Height Humulus Hydrofluoric Acid Hydrogen Peroxide Hydrophobic Surfaces In Vitro Individual Knowledge Libraries Logic Maps Measurement Measures Metals Methods Mind Modeling Molds Molecular Conformation Motion Names Nature Optics Osteoblasts Paint Pattern Personal Satisfaction Physical shape Plant Roots Play Polymers Polystyrenes Positioning Attribute Preparation Printing Process Property Range Reporting Research Rest Role Room, Clean Rupture Sampling Scanning Semiconductors Signal Transduction Silicon Silicones Solutions Solvents Staging Steel Structure Surface Surface Tension System Tail Techniques Temperature Thick Thiophene Thiophenes Transistors Variant Viscosity biomaterial compatibility bone bone cell cell behavior cell growth combinatorial cytotoxic design desire electric field falls healing in vivo instrument interest monomer nanolithography nanopattern oxidation particle poly(3-octylthiophene)polypyrrole polythiophene research study response sensor size

项目摘要

DESCRIPTION (provided by applicant): Investigating cellular response to biomaterials is of great interest, particularly the interaction with biomaterials that incorporate stimulatory cues, such as electric signals, to regulate cell functions. Biomaterials could also be engineered to respond to cellular behavior, thus potentially applicable in the development of diagnostic sensors. It has been demonstrated that biomaterial surface topography, chemical nature, and stimulatory characteristics influence cell behavior. For example, it has been shown that osteoblasts (bone-forming cells) respond to electrical stimulation as well as to substrate topography. The large variable space and complex phenomena that control materials surface features encourages the use of high-throughput methods to evaluate them. With this in mind, the overall objective of this proposal is to develop combinatorial patterned libraries of conductive polythiophene and nonconductive polystyrene as substrates for inducing attachment and proliferation in osteoblasts. The combinatorial approach will allow us to create surfaces with thousands of variations in properties allowing efficient study of the effect of polymer surface features and chemistry on cell functions.

描述（由申请人提供）：研究对生物材料的细胞反应引起了人们的极大兴趣，尤其是与结合刺激性提示的生物材料的相互作用，例如电信号，以调节细胞功能。也可以设计生物材料以应对细胞行为，因此可能适用于诊断传感器的开发。已经证明生物材料表面形貌，化学性质和刺激特征会影响细胞行为。例如，已经表明成骨细胞（骨形成细胞）对电刺激以及底物地形响应。控制材料表面特征的大型可变空间和复杂现象鼓励使用高通量方法来评估它们。考虑到这一点，该提案的总体目标是开发导电性聚噻吩和非导导性聚苯乙烯的组合图案库，作为用于在成骨细胞中诱导附着和增殖的底物。组合方法将使我们能够创建具有数千种属性变化的表面，从而有效研究聚合物表面特征和化学对细胞功能的影响。