Spore Dispersal and Germination in Stachybotrys

水穗属植物中的孢子传播和萌发

• 批准号: 7413786
• 负责人: NICHOLAS P MONEY
• 金额: $ 3.5万
• 依托单位: MIAMI UNIVERSITY OXFORD
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7413786
• 关键词: Age; Air; American; Attention; Biological; Biology; Condition; Country; Deposition; Environment; Exposure to; Germination; Hemorrhage; Home environment; Hospitals; Hypersensitivity; Infant; Insecta; Laboratories; Lung; Mechanics; Methods; Molds; Mycotoxins; Neurologic; Ohio; Proteins; Public Health; Reporting; Reproduction spores; Research; Research Personnel; Stachybotrys; Students; Surface; Temperature; Toxin; Travel; Trichothecenes; Water; Water Movements; Work; Workplace; clinically significant; density; design; experience; exposed human population; fungus; injured; research study; respiratory; vector; vibration

This project is concerned with spore dispersal in the black mold Stachybotrys chartarum, a toxin-producing fungus of growing public health concern as a contaminant of water-damaged buildings. Human exposure to its spores has been associated with a variety of respiratory and neurological illnesses, including a cluster of idiopathic pulmonary hemorrhage (IPH) cases in infants in Ohio. The biological activity of Stachybotrys toxins is a subject of intensive research effort in other laboratories, but little attention has been paid to the mechanisms of spore dispersal that cast these compounds into the air. This project is motivated by the following question: How do the sticky spores of this mold move and contaminate the indoor environment? The first set of experiments will examine the effects of airflow, substrate vibration, and water movement on spore dispersal. The possibility that insects may act as vectors for dispersal of Stachybotrys spores will be the focus of a second aim. Thirdly, spore germination will be assessed using a multi-well plate method in relation to water availability, substrate composition, temperature, conidial age, and density of conidial deposits. These experiments will clarify both the mode of human exposure (an issue of major clinical significance), and the way that the mold spreads through buildings. Many of the proposed experiments are perfectly suited for undergraduate students. Students involved in this work will benefit by gaining extremely valuable laboratory experience, and by making important contributions to our understanding of the biology of Stachybotrys.

该项目涉及产毒素的黑霉菌Stachyboboestrumchartarum的孢子传播 真菌作为水破坏建筑物的污染物，日益引起公众健康的关注。人类接触 它的孢子与多种呼吸道和神经系统疾病有关，包括一群 俄亥俄州婴儿特发性肺出血病例。水苏毒素的生物活性 是其他实验室密集研究的主题，但很少注意到 将这些化合物抛入空气中的孢子传播机制。该项目的动机是 问题：这种霉菌的粘性孢子是如何移动并污染室内环境的？ 第一组实验将检查气流、基底振动和水运动对 孢子散布昆虫可能作为传播水葫芦孢子的媒介， 第二个目标的焦点。第三，将使用多孔板方法评估孢子萌发， 与水分有效性、基质成分、温度、分生孢子年龄和分生孢子密度有关 存款这些实验将阐明人类暴露的模式（一个主要的临床问题）和 重要性），以及霉菌在建筑物中传播的方式。许多提议的实验是 非常适合本科生。参与这项工作的学生将受益于获得极大的 宝贵的实验室经验，并作出重要贡献，我们的生物学的理解， 水苏属