Core--Clinical resources

描述(由申请人提供)： 核心A将作为所有项目和核心的资源。它的本金 目标是招募并重新审查我们最初队列中的950名成员 圣安东尼奥家庭心脏研究的参与者。考试将会进行 在弗雷德里克·C·巴特综合临床研究中心 位于圣安东尼奥的奥迪·墨菲退伍军人纪念医院。这个 临床检查将包括四个要素：核实和同意；以及 采访详细介绍了病史、生育史、药物和维生素 使用情况和生活方式行为；体格测量(心率、血液 压力，以及对肥胖的各种评估)；以及静脉切开术。数据和 血液样本将被送往西南生物医学基金会 研究核心A人员将在哪里：编辑血样；进行生化 血糖、胰岛素、胆固醇和甘油三酯浓度的测量 和脂蛋白大小表型；并检查所有数据的真实性和 在将数据文件提供给程序调查人员之前，确保数据文件的一致性。 (摘要结束) 由Rainwater博士领导的临床核心对 数据和样本的收集、样本的处理和存储、 从血液样本和数据中测量大量的表型 录入和验证。在这次风暴中，共有809个个体被发现 计划项目；950人将在前四个月被带回 多年的SAFHS3。考试将在奥迪区的GCRC举行 墨菲纪念退伍军人医院和所有检查用品将 由GCRC提供。核心A员工将负责数据管理 和样品处理。调查人员已经证明，他们有能力 在后续研究中得到家庭成员的良好参与，并将 加倍努力跟踪重要的血统成员。一组类似的电池 调查问卷和测量方法将与上一次一样使用 学习。调查人员已决定不再跟进家庭信息 为了时间的利益。或许至少有必要评估一下 未返回的个人的状况和健康状况。它也是一种 遗憾的是，颈动脉测量在一年内无法再次获得 纵向研究，特别是考虑到初始遗传力如此之强 然而，这可能会非常昂贵和耗时。 这个核心对整个项目的成功至关重要，就像新的样本一样 脂肪组织的内分泌功能所需的变化 项目1的表型和项目2的氧化应激表型。 样品处理、样品储存、脂蛋白分析的通用规程 和质量控制都很好。 需要更多关于处理新的样品的协议的信息 需要测量的表型以及如何将样本转移到外部实验室 并将完成信息的返回。这个核心的一个关键优势 是与家属和诊所工作的人员的连续性。

DESCRIPTION (provided by the applicant): Core A will serve as a resource for all projects and cores. Its principal objective is to recruit and re-examine 950 members of our original cohort of participants in the San Antonio Family Heart Study. The examination will take place in the Frederick C. Bartter General Clinical Research Center which is located at the Audie Murphy Memorial Veterans Hospital in San Antonio. The clinic examination will consist of four elements: verification and consent; an interview detailing medical and reproductive history, medications and vitamin usage, and lifestyle behaviors; physical measurements (heart rate, blood pressure, and various assessments of adiposity); and phlebotomy. The data and blood samples will be transported to Southwest Foundation for Biomedical Research where Core A personnel will: edit blood samples; conduct biochemical measurements of glucose, insulin, cholesterol and triglyceride concentrations and lipoprotein size phenotypes; and check all data for plausibility and consistency before making the data files available to program investigators. (End of abstract) The Clinical Core led by Dr. Rainwater has primary responsibility for the collection of data and samples, processing and storage of samples, the measurement of a large number of phenotypes from the blood samples and data entry and validation. A total of 809 individuals were seen during the current program project; 950 individuals will be brought back during the first four years of SAFHS3. The examinations will take place in the GCRC in the Audie Murphy Memorial Veterans Hospital and all supplies for the examination will be provided by the GCRC. Core A staff will be responsible for data management and sample handling. The investigators have shown that they are capable of getting good participation from family members in the follow-up study and will make extra efforts to follow key pedigree members. A similar battery of questionnaires and measurements will be used as was used in the previous study. The investigators have decided not to follow-up on family information in the interest of time. It might be worthwhile to at least assess vital status and health status of individuals who are not returning. It is also a shame that carotid artery measurements cannot be obtained again for a longitudinal study, especially since the initial heritabilities were so strong however this would likely be very costly and time consuming. This core is vital to the success of the project as a whole as new samples are required for the endocrinological function of adipose tissue and change in phenotypes in project 1 and the oxidative stress phenotypes in project 2. The general protocols for sample handling, sample storage, lipoprotein analysis and quality control are excellent. More information is needed on protocols for handling samples for the new phenotypes to be measured and how transfer of samples to outside laboratories and return of information will be accomplished. A key strength of this core is the continuity of personnel working with the families and in the clinic.