Histopathology Core

组织学核心将使用标准染色和免疫组织化学评价组织切片， 免疫印迹法、组织制备和分配用于毒理学研究和生物分布研究 在项目1和2中描述的mdx小鼠和恒河猴中进行的实验研究。 由于以下原因，历史学核心对U54提案的成功至关重要： 1)每个项目的使命都将由一个具有内置质量控制的实验室的专业知识来促进 以一致的方式处理所有组织的措施。 2)通过将PI与结果测量分开，将增强结果的客观性。 3)通过将所有组织切片处理为 同样的工作人员。 4)Hierarchology Core中可用的技术并不适用于每个项目中的所有Pi。 组织学实验室将评估所有项目的基因转移后的肌肉， 项目之间的一致性。

The Histopathology Core will evaluate histologic sections using standard stains and immunohistochemistry, western blots, tissue preparation and allocation for toxicology studies and biodistribution studies from all experimental studies done in mdx mice and Rhesus macaques monkeys described in Projects 1 and 2. The Histopathology Core is critical to the success of this U54 proposal for the following reasons: 1) The mission of each Project will be facilitated by the expertise of one lab with built in quality control measures to process all tissues in a consistent manner. 2) The objectivity of the results will be enhanced by separating the Pi's from the outcome measures. 3) There will be greater consistency between each of the Projects by having all tissue sections processed by the same staff. 4) Techniques available in the Histopathology Core are not common to all Pi's in each project. The Histopathology Laboratory will evaluate muscle following gene transfer from all projects to maintain consistency between projects.