Histopathology Core

组织病理学核心

• 批准号: 8130727
• 负责人: Zarife Sahenk
• 金额: $ 25.92万
• 依托单位: RESEARCH INST NATIONWIDE CHILDREN'S HOSP
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8130727
• 关键词: Biodistribution; Blood Vessels; Cells; Child; Computer software; Dyes; Evans blue stain; Fluorescence; Formalin; Gastrocnemius Muscle; Gene Expression; Gene Transfer; Histologic; Histopathology; Immune; Immunohistochemistry; Immunosuppressive Agents; Laboratories; Laboratory Research; Macaca mulatta; Measures; Membrane; Methods; Microscope; Microtome - medical device; Mission; Molecular; Monkeys; Mononuclear; Morphology; Mus; Muscle; Outcome Measure; Paraffin; Pathology; Physiological; Polypeptide N-acetylgalactosaminyltransferase; Process; Property; Proteins; Quality Control; Regimen; Reporting; Research Institute; Research Personnel; Resources; Routine Histology Staining Method; Sampling; Services; Staining method; Stains; Techniques; Testing; Tissue Sample; Tissues; Toxicology; Transferase; Transgenes; Viral Genome; Western Blotting; Work; base; experience; extensor digitorum; gene therapy; instrument; mdx mouse; micro-dystrophin; research study; success; tibialis anterior muscle; tissue preparation; tissue processing; transgene expression; uptake

The Histopathology Core will evaluate histologic sections using standard stains and immunohistochemistry, western blots, tissue preparation and allocation for toxicology studies and biodistribution studies from all experimental studies done in mdx mice and Rhesus macaques monkeys described in Projects 1 and 2. The Histopathology Core is critical to the success of this U54 proposal for the following reasons: 1) The mission of each Project will be facilitated by the expertise of one lab with built in quality control measures to process all tissues in a consistent manner. 2) The objectivity of the results will be enhanced by separating the Pi's from the outcome measures. 3) There will be greater consistency between each of the Projects by having all tissue sections processed by the same staff. 4) Techniques available in the Histopathology Core are not common to all Pi's in each project. The Histopathology Laboratory will evaluate muscle following gene transfer from all projects to maintain consistency between projects.

组织病理学核心将使用标准染色和免疫组织化学来评估组织切片， 来自ALL的毒理学研究和生物分布研究的蛋白质印迹、组织制备和分配 在项目1和2中描述的在MDX小鼠和猕猴身上进行的实验研究。 组织病理学核心对U54提案的成功至关重要，原因如下： 1)每个项目的任务将由一个具有内置质量控制的实验室的专业知识来促进 以一致的方式处理所有组织的措施。 2)通过将PI从结果度量中分离出来，将提高结果的客观性。 3)每个项目之间的一致性将更大，因为所有组织切片都由 同样的手杖。 4)组织病理学核心中提供的技术并不是每个项目中所有PI都通用的。 组织病理学实验室将评估所有项目的基因转移后的肌肉，以保持 项目之间的一致性。