Histopathology core

组织病理学核心

基本信息

项目摘要

The Histopathology Core will perform a vital function in Paul D. Weilstone MDCRC. The director of the core is an experienced muscle pathologist (Z. Sahenk, MD, Ph.D, Director of the Histopathology Laboratory at Nationwide Children's Hospital). Project 1 will require analysis of muscle tissue taken directly from DMD patients. In Aim 1 of Project 1, T cell infiltration will be examined as an expression of a pre-existing immune response to dystrophin. In addition, sequencing of the DMD mRNA will be performed, in order to assess the relationship between altered splicing and antigenic dystrophin epitopes. In Aim 2 of Project 1, specific T cell immunity will be studied before and after corticosteroid treatment. Aim 3 of Project 1 closely scrutinizes gene expression and immune response following AAV-micro-dystrophin delivery to muscle. In Project 2 emphasis switches to an experimental paradigm using the rhesus macaque. This too is familiar territory to this laboratory because tissue from this animal model has been processed in this laboratory for the past several years. In Aim 1 of project 1 AAV.micro-dystrophin gene delivery will be examined after depleting neutralizing antibodies to study the role of T cell immunity to viral capsid. In Aim 2 of project 2 tissue sections will be used to help define the role of CD4+ and CD8+ T cells against a foreign transgene product. In the final Aim 3 of Project 2 similar tissue studies will be necessary to understand the role of possible immunosuppressant agents. In the course of study of both human and non-human primate tissue, histologic sections will employ standard stains and immunohistochemistry for gene expression and Immunological markers for T cell subsets, and western blots similar to every day studies in this laboratory. The success of this proposal is dependent on the experience and excellence ofthis laboratory for the following reasons: 1) The mission of each Project will be facilitated by the expertise of a single laboratory with built in quality control measures to process all tissues in a consistent manner. 2) The objectivity of the results will be enhanced by separating the Pi's in Projects 1 and 2 from the outcome measures. 3) There will be greater consistency between each of the Projects by having all tissue sections processed by the same staff.
组织病理学核心将在Paul D. Weilstone MDCRC中发挥重要作用。中心主任是一位经验丰富的肌肉病理学家(Z. Sahenk,医学博士,全国儿童医院组织病理学实验室主任)。项目1将需要分析直接取自DMD患者的肌肉组织。在项目1的目的1中,T细胞浸润将被检查为对肌营养不良蛋白预先存在的免疫反应的表达。此外,将对DMD mRNA进行测序,以评估剪接改变与抗原性肌营养不良蛋白表位之间的关系。在Project 1的Aim 2中,将研究皮质类固醇治疗前后的特异性T细胞免疫。项目1的目的3密切关注aav -微肌营养不良蛋白递送至肌肉后的基因表达和免疫反应。在项目2中,重点转向使用恒河猴的实验范式。这也是本实验室熟悉的领域因为这个动物模型的组织在过去几年里一直在本实验室处理。在AAV项目1的目标1中。将在耗尽中和抗体后检测微肌营养不良蛋白基因的传递,以研究T细胞免疫对病毒衣壳的作用。在项目2的目标2中,组织切片将用于帮助定义CD4+和CD8+ T细胞对抗外来转基因产物的作用。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Zarife Sahenk其他文献

Zarife Sahenk的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Zarife Sahenk', 18)}}的其他基金

NT-3 gene therapy to improve peripheral nerve function induced by genetic defect
NT-3基因疗法改善遗传缺陷引起的周围神经功能
  • 批准号:
    8132939
  • 财政年份:
    2010
  • 资助金额:
    $ 15.99万
  • 项目类别:
NT-3 gene therapy to improve peripheral nerve function induced by genetic defect
NT-3基因疗法改善遗传缺陷引起的周围神经功能
  • 批准号:
    8333412
  • 财政年份:
    2010
  • 资助金额:
    $ 15.99万
  • 项目类别:
NT-3 gene therapy to improve peripheral nerve function induced by genetic defect
NT-3基因疗法改善遗传缺陷引起的周围神经功能
  • 批准号:
    7988407
  • 财政年份:
    2010
  • 资助金额:
    $ 15.99万
  • 项目类别:
Histopathology core
组织病理学核心
  • 批准号:
    8032770
  • 财政年份:
    2010
  • 资助金额:
    $ 15.99万
  • 项目类别:
Histopathology Core
组织病理学核心
  • 批准号:
    7328079
  • 财政年份:
    2007
  • 资助金额:
    $ 15.99万
  • 项目类别:
Histopathology core
组织病理学核心
  • 批准号:
    8685781
  • 财政年份:
  • 资助金额:
    $ 15.99万
  • 项目类别:
Histopathology Core
组织病理学核心
  • 批准号:
    7907723
  • 财政年份:
  • 资助金额:
    $ 15.99万
  • 项目类别:
Histopathology Core
组织病理学核心
  • 批准号:
    8130727
  • 财政年份:
  • 资助金额:
    $ 15.99万
  • 项目类别:
Histopathology core
组织病理学核心
  • 批准号:
    8473900
  • 财政年份:
  • 资助金额:
    $ 15.99万
  • 项目类别:
Histopathology core
组织病理学核心
  • 批准号:
    8377177
  • 财政年份:
  • 资助金额:
    $ 15.99万
  • 项目类别:

相似海外基金

Quantification of Neurovasculature Changes in a Post-Hemorrhagic Stroke Animal-Model
出血性中风后动物模型中神经血管变化的量化
  • 批准号:
    495434
  • 财政年份:
    2023
  • 资助金额:
    $ 15.99万
  • 项目类别:
Bioactive Injectable Cell Scaffold for Meniscus Injury Repair in a Large Animal Model
用于大型动物模型半月板损伤修复的生物活性可注射细胞支架
  • 批准号:
    10586596
  • 财政年份:
    2023
  • 资助金额:
    $ 15.99万
  • 项目类别:
A Comparison of Treatment Strategies for Recovery of Swallow and Swallow-Respiratory Coupling Following a Prolonged Liquid Diet in a Young Animal Model
幼年动物模型中长期流质饮食后吞咽恢复和吞咽呼吸耦合治疗策略的比较
  • 批准号:
    10590479
  • 财政年份:
    2023
  • 资助金额:
    $ 15.99万
  • 项目类别:
Small animal model for evaluating the impacts of cleft lip repairing scar on craniofacial growth and development
评价唇裂修复疤痕对颅面生长发育影响的小动物模型
  • 批准号:
    10642519
  • 财政年份:
    2023
  • 资助金额:
    $ 15.99万
  • 项目类别:
Diurnal grass rats as a novel animal model of seasonal affective disorder
昼夜草鼠作为季节性情感障碍的新型动物模型
  • 批准号:
    23K06011
  • 财政年份:
    2023
  • 资助金额:
    $ 15.99万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Longitudinal Ocular Changes in Naturally Occurring Glaucoma Animal Model
自然发生的青光眼动物模型的纵向眼部变化
  • 批准号:
    10682117
  • 财政年份:
    2023
  • 资助金额:
    $ 15.99万
  • 项目类别:
A whole animal model for investigation of ingested nanoplastic mixtures and effects on genomic integrity and health
用于研究摄入的纳米塑料混合物及其对基因组完整性和健康影响的整体动物模型
  • 批准号:
    10708517
  • 财政年份:
    2023
  • 资助金额:
    $ 15.99万
  • 项目类别:
A Novel Large Animal Model for Studying the Developmental Potential and Function of LGR5 Stem Cells in Vivo and in Vitro
用于研究 LGR5 干细胞体内外发育潜力和功能的新型大型动物模型
  • 批准号:
    10575566
  • 财政年份:
    2023
  • 资助金额:
    $ 15.99万
  • 项目类别:
Elucidating the pathogenesis of a novel animal model mimicking chronic entrapment neuropathy
阐明模拟慢性卡压性神经病的新型动物模型的发病机制
  • 批准号:
    23K15696
  • 财政年份:
    2023
  • 资助金额:
    $ 15.99万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
The effect of anti-oxidant on swallowing function in an animal model of dysphagia
抗氧化剂对吞咽困难动物模型吞咽功能的影响
  • 批准号:
    23K15867
  • 财政年份:
    2023
  • 资助金额:
    $ 15.99万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了