Core--Transgenic Mice

核心--转基因小鼠

• 批准号: 7406852
• 负责人: Mary E Fabry
• 金额: $ 13.76万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7406852
• 关键词: Animals; Breeding; Cell Adhesion; Epigenetic Process; Erythrocytes; Factor VIII-Related Antigen; Genetic Epistasis; Hemoglobin; High Pressure Liquid Chromatography; Human; Knock-out; Mus; P-Selectin; Patients; Proteins; Research Personnel; Services; Sickle Cell; TNFRSF5 gene; Transgenic Mice; Transgenic Organisms; VWF gene; density; design; pleiotropism; polymerization; research study; sickling; von Willebrand Factor

The animal core will breed transgenic mice which will be identified and characterized for use in Project 1:Design of alpha-chains that fully neutralize polymerization; Project 2: MRl and NIRS in SS patients and mice; Project 3: Sickle Cell Adhesion; and Project 4: Sickle vasocclusion: pleiotropism, epistasis, epigenetics. Our function will be mainly to provide assistance to investigators in the marking, identification, and record keeping of founders and their offspring. These mice will include the NY1KO mice that express exclusively human hemoglobins: HbS and three different levels of HbF; the BERK mice (originally described by Paszty et al) and BERK mice expressing HbF. Knockouts for iNOS, nNOS, vWF, and P-selectin will be bred into our sickle lines as will a variety of knockout and transgenic lines described in Project 4. Also included are mice that express exclusively HbC and exclusively HbA, as well as suitable control mice for all of the proposed experiments. This core also provides analytic services for other projects: protein identification and quantitation (HPLC, IEF, mass spec), red cell characterization (p50, intracellular polymerization, 2,3-DPG analysis), and red cell density distribution.

动物核心将培育转基因小鼠，其将被鉴定和表征以用于项目1：完全中和聚合的α链的设计;项目2：SS患者和小鼠中的MRI和NIRS;项目3：镰状细胞粘附;和项目4：镰状血管闭塞：多效性、上位性、表观遗传学。我们的职能主要是协助调查人员对创始人及其后代进行标记、识别和记录保存。这些小鼠将包括仅表达人血红蛋白HbS的NY 1 KO小鼠 和三种不同水平的HbF; BERK小鼠（最初由Paszty等人描述）和表达HbF的BERK小鼠。iNOS、nNOS、vWF和P-选择素的敲除将被培育到我们的镰状系中，项目4中描述的各种敲除和转基因系也将被培育到我们的镰状系中。还包括专门表达HbC和专门表达HbA的小鼠，以及用于所有提出的实验的合适的对照小鼠。该核心还为其他项目提供分析服务：蛋白质鉴定和定量（HPLC，IEF，质谱），红细胞表征（p50，细胞内聚合，2，3-DPG分析）和红细胞密度分布。