3D functional and structural imaging of cardiac tissue

心脏组织的 3D 功能和结构成像

• 批准号: 7273658
• 负责人: Christine P Hendon
• 金额: $ 3.86万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7273658
• 关键词: Action Potentials; Arrhythmia; Biological; Cardiac; Cell model; Coupling; Data; Depth; Development; Dimensions; Dyes; Fluorescence; Fluorescence Microscopy; Functional Imaging; Generations; Heterogeneity; Human; Image; Maps; Microelectrodes; Microscopy; Modality; Modeling; Morphology; New Zealand; Optical Coherence Tomography; Optics; Oryctolagus cuniculus; Pattern; Preparation; Resolution; Sampling; Signal Transduction; Structure; Structure-Activity Relationship; System; Technology; Testing; Thinking; Three-Dimensional Imaging; Tissues; atrioventricular node; base; millimeter; new technology; second harmonic; three dimensional structure; voltage

DESCRIPTION (provided by applicant): Abnormalities of cellular or tissue heterogeneity are thought to provide a mechanism for many forms of human arrhythmias. The objective of this project is to develop technologies that will correlate the structural and functional relationships of cardiac tissue morphology and conduction heterogeneities. This project will provide an enhancement to viewing the conduction of action potentials within cardiac tissue in three dimensions. By coupling functional imaging with structural imaging, three dimensional structural imaging and depth information of the action potentials can be acquired simultaneously. With this new system, we can test the hypothesis if there is a structural basis for the heterogeneity of action potential velocities and morphology in the AV node. Isolated AV node preparations from New Zealand White Rabbits will be used as a model in this project. Other models of cellular or tissue heterogeneity can be imaged with this system to correlate the structure-function relationship in substrates of arrhythmias.

描述（由申请人提供）：认为细胞或组织异质性的缺失为多种形式的人类心律失常提供了机制。本项目的目标是开发将心脏组织形态和传导异质性的结构和功能关系相关联的技术。这个项目将提供一个增强查看心脏组织内的三维动作电位传导。通过将功能成像与结构成像相结合，可以同时获得动作电位的三维结构成像和深度信息。有了这个新的系统，我们可以测试的假设，如果有一个结构基础的动作电位速度和形态的异质性在房室结。新西兰白色家兔的分离房室结制备物将用作本项目的模型。细胞或组织异质性的其他模型可以用该系统成像，以关联心律失常基质中的结构-功能关系。