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Microarrays in Fathead Minnows and Bass

黑头鱼和鲈鱼的微阵列

基本信息

批准号：
7208050
负责人：
BARBARA JOAN CARTER
金额：
$ 38.59万
依托单位：
ECOARRAY, INC.
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-03-15 至 2008-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7208050
关键词：
11-ketotestosterone Academia Address Affect Androgen Antagonists Androgen Receptor Androgens Animal Model Animals Aromatase Aryl Hydrocarbon Receptor Atrazine Bass Binding Biochemical Biological Biological Assay Biology Cadmium Carbon Tetrachloride Case Study Chemistry Client Collaborations Cooperative Research and Development Agreement Cytochrome P450 Data Data Analyses Databases Development Diagnostic End Point Environment Environmental Exposure Environmental Monitoring Environmental Pollution Estrogen Receptors Estrogens Ethinyl Estradiol Evaluation Exposure to Farming environment Fertility Financial compensation Fingerprint Fishes Florida Gene Chips Gene Expression Genes Genetic Genomics Glass Goals Government Grant Health Human In Vitro Individual Industry Insecticides Knowledge Laboratories Lead Licensing Link Market Research Marketing Measures Mercury Metabolism Metals Methods Minnows Mixed Function Oxygenases Molecular Molecular Profiling Monitor Numbers Oligonucleotides Organism Pathway interactions Pattern Pharmacologic Substance Phase Physiological Physiology Population Positioning Attribute Procedures Protocols documentation Publications Purpose RNA Range Reagent Reproductive Health Research Research Personnel Risk Assessment Sampling Scientist Screening procedure Sentinel Services Site Solid Spottings Standards of Weights and Measures System Technology Testing Thinking Tissues Today Toxaphene Toxic effect Toxicology Trenbolone Trichloroethylene United States Environmental Protection Agency Validation Vertebral column Water Weight Work base bisphenol A cDNA Library commercial application commercialization egg environmental toxicology field study gene discovery human disease in vitro Assay in vivo interest manufacturing process mortality organochlorine pesticide phenanthrene posters programs prototype remediation reproductive response success superfund site tool

项目摘要

DESCRIPTION (provided by applicant): Microarrays are a powerful way to measure the biological impact of contaminants in the environment. Because they are used to test changes in expression of many genes in parallel, they provide data on the mechanisms by which compounds affect health more quickly than methods now in use, which makes them much less expensive than current tests. Today, gene chips (microarrays) are an established technology in human disease research. The purpose of the Phase I grant we just completed and this proposed continuation is to bring their power and precision to environmental toxicology. By understanding how contaminants affect gene expression in fish and other wildlife, we can begin to link environmental exposures to adverse health effects in these animals and by extrapolation, to humans. In this Phase II proposal, EcoArray intends to develop, validate and begin to market large oligonucleotide-based glass microarrays for fathead minnows and largemouth bass and develop the backbone of a powerful database. In Phase III, we will use knowledge obtained in this grant to finalize our product offering of a suite of general- and special-purpose microarrays for research and environmental monitoring by Academia, Government and Industry. Our successful Phase I grant validated the use of arrays for a small set of environmental contaminants and resulted in prototype arrays for the two species, as described in our publications, presentations and posters. Our goal for this Phase II work is to expand the current arrays to a larger, more robust format, resulting in a 10,000+-gene fathead minnow microarray and 2,000+ gene largemouth bass microarray. We have engaged five collaborators, all of whom are experts in fish biology, to carry out exposures and field analysis in fathead minnows or largemouth bass. For the fathead minnow studies, we will use the microarrays that we will develop to identify and characterize the genetic signatures for 14 different compounds commonly found at superfund sites, and that are known to have distinct modes of action. Many of these compounds are on the 2003 Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) Priority list. We are currently involved in a collaboration (CRADA) with the EPA to pick the best microarray platform and validate its use, and this commercial platform will be the basis for our enhanced microarrays. We will incorporate the gene expression data into a relational database that will also include other parameters such as water chemistry data and biological and physiological endpoints in the treatment species. We will conduct similar laboratory and field studies, but on a smaller scale, in largemouth bass and identify and characterize the genetic signatures for 5 different compounds. We have begun our marketing plan, and have had early success in selling laboratory services associated with gene discovery. We expect to offer an oligonucleotide-based chip developed with the EPA in late 2005 and then begin our marketing launch of the chips developed in this grant, as well as several other complementary products, in late 2006.

描述（由申请人提供）：微阵列是测量环境中污染物生物影响的有效方法。因为它们被用来平行测试许多基因表达的变化，它们提供了关于化合物比现在使用的方法更快地影响健康的机制的数据，这使得它们比目前的测试便宜得多。今天，基因芯片（微阵列）是人类疾病研究中的一项成熟技术。我们刚刚完成的第一阶段拨款的目的是将它们的力量和精度带到环境毒理学中。通过了解污染物如何影响鱼类和其他野生动物的基因表达，我们可以开始将环境暴露与这些动物的不良健康影响联系起来，并通过外推将其与人类联系起来。在这个第二阶段的建议，生态阵列打算开发，验证和开始市场的大规模基于荧光素的玻璃微阵列的黑头呆鱼和大嘴鲈鱼和开发一个强大的数据库的骨干。在第三阶段，我们将利用这笔赠款获得的知识，最终确定我们的一套通用和专用微阵列产品，用于学术界，政府和工业界的研究和环境监测。我们成功的第一阶段资助验证了阵列用于一小部分环境污染物，并为两个物种提供了原型阵列，如我们的出版物，演示文稿和海报所述。我们的第二阶段工作的目标是将目前的阵列扩展到更大，更强大的格式，从而产生10，000+基因的黑头呆鱼微阵列和2，000+基因的大嘴鲈鱼微阵列。我们聘请了五位合作者，他们都是鱼类生物学专家，对黑头呆鱼或大口黑鲈进行暴露和实地分析。对于黑头呆鱼的研究，我们将使用我们将开发的微阵列来识别和表征14种不同化合物的遗传特征，这些化合物通常在超级基金网站上发现，并且已知具有不同的作用模式。这些化合物中的许多都在2003年综合环境反应，赔偿和责任法案（CERCLA）的优先名单上。我们目前正在与EPA合作（CRADA），以挑选最佳的微阵列平台并验证其使用，这个商业平台将成为我们增强微阵列的基础。我们将把基因表达数据纳入一个关系数据库，该数据库还将包括其他参数，如水化学数据以及处理物种的生物和生理终点。我们将进行类似的实验室和实地研究，但规模较小，在大口黑鲈和确定和表征5种不同化合物的遗传特征。我们已经开始我们的营销计划，并在销售与基因发现有关的实验室服务方面取得了初步成功。我们预计将在2005年底提供与EPA开发的基于谷胱甘肽的芯片，然后在2006年底开始我们在该赠款中开发的芯片以及其他几种互补产品的市场推广。