Rapid LSPR detection of cardiac markers

描述（由申请人提供）：心血管疾病是美国和其他发达国家的头号杀手。许多急性心肌梗死或心脏病发作的患者不能活着到达医院，而一些活着的人一旦到达医院就没有活下来。在诊断和治疗心脏病发作时，时间是至关重要的，尽管有有效的治疗方法，如原发性血管成形术、纤维溶栓和抗血栓治疗，但这些治疗方法只有在确认心脏病发作后才能开始。确认心脏病发作的能力是建立在不够快速或明确的技术基础上的，即使迄今为止取得了许多医学进步，也需要更有效和准确的诊断，以便为患者提供适当和更快的治疗。对许多患者来说，使用心脏标志物进行诊断是至关重要的。现有的生物标志物检测分析方法耗时费力，成本高，需要复杂的设备，并且不适合快速诊断。迫切需要新的分析方法，提供快速和可靠的鉴定痕量生物标志物。基于局域表面等离子体共振（LSPR）光谱的光学生物传感器可以满足这一需求，提供低皮摩尔到高飞摩尔浓度范围的检测。然而，LSPR传感器的商业化和广泛使用受到缺乏高度均匀、可重复和廉价的传感器平台的阻碍，这些平台具有特定的目前未知的几何参数。提出的多阶段SBIR项目旨在开发和商业化新型纳米结构LSPR传感器平台，该平台利用高度有序的均匀平行金属纳米线阵列固定在自组织纳米模板中。第一阶段的目标是通过制造和测试c反应蛋白（一种急性心肌梗死标志物）的生物传感器来证明所提出方法的可行性。该方法将使广泛的便携式传感器系统和诊断套件能够对各种心血管疾病、癌症、糖尿病和传染病、医疗保健、消费者和工业环境监测、农业和食品部门以及国防和国土安全应用中的生物标志物进行特异性和敏感的检测，以可靠地快速诊断。该项目解决了公共卫生保护中最重要的问题之一：对心脏病发作和其他疾病进行可靠的明确诊断，以便向患者提供适当和更快的治疗。提出了用于检测生物标志物的新型先进传感器-特定疾病的分子特征。拟议的传感技术将使广泛的便携式传感器系统和诊断套件能够有效和准确地诊断各种心血管疾病、癌症、糖尿病和传染病，并用于检测医疗保健、消费者和工业环境监测、农业和食品部门以及国防和国土安全应用中的感兴趣的生物分子、化学制剂和生物危害。

DESCRIPTION (provided by applicant): Cardiovascular disease is the number one killer in the United States and in other developed countries. Many victims of acute myocardial infarction, or heart attack, do not make it to the hospital alive, and a number of those who do don't survive once they get there. Time is of the essence in diagnosing and treating heart attacks, and although there are effective treatments such as primary angioplasty, fibrionolysis, and anti-thrombotic therapy - those treatments cannot be initiated until a heart attack is confirmed. The ability to confirm a heart attack is based on techniques that are not sufficiently rapid or definitive, and even with the many medical advances that have been made to date, there is a need for more efficient and accurate diagnosis in order to provide proper and quicker treatment to the patient. For many patients, use of cardiac markers for diagnosis is critical. Available analytical methods for biomarkers detection are labor- and time consuming, costly, require sophisticated equipment, and are not suitable for express diagnosis. There is a pressing need for new analytical methods that provide rapid and reliable identification of trace biomarkers. Optical biosensors based on Localized Surface Plasmon Resonance (LSPR) spectroscopy, providing detection in the low picomolar to high femtomolar concentration range, could fill this need. However, commercialization and wide use of LSPR sensors is impeded by the absence of highly uniform, reproducible, and inexpensive sensor platform with specific presently unknown geometric parameters. The proposed multi-phase SBIR project targets the development and commercialization of novel nanostructured LSPR sensor platform utilizing a highly ordered array of uniform parallel metal nanowires anchored in a self-organized nanotemplate. The Phase I objective is to demonstrate feasibility of the proposed approach by fabricating and testing a biosensor for C-Reactive Protein - one of the acute myocardial infarction markers. The approach will enable a broad family of portable sensor systems and diagnostic kits for specific and sensitive detection of biomarkers for reliable express diagnosis of various cardiovascular diseases, cancer, diabetes, and infectious diseases, biological molecules in health care, consumer and industry-based environmental monitoring, agricultural and food sectors, and in defense and homeland security applications. The project addresses one the most significant problems in public health protection: reliable express diagnosis of a heart attack and other diseases in order to provide proper and quicker treatment to the patient. The development of novel advanced sensors for detection of biomarkers - molecular signatures of a specific disease - is proposed. The proposed sensing technology will enable a broad family of portable sensor systems and diagnostic kits for efficient and accurate diagnosis of various cardiovascular diseases, cancer, diabetes, and infectious diseases, and for the detection of biological molecules of interest, chemical agents and biohazards in health care, consumer and industry-based environmental monitoring, agricultural and food sectors, and in defense and homeland security applications.