Highly bright and narrow emitting nanocrystal fluoreagents for multiplexed detect

用于多重检测的高亮度和窄发射纳米晶体荧光剂

• 批准号: 7325862
• 负责人: YUNJUN WANG
• 金额: $ 10万
• 依托单位: MESOLIGHT, LLC
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2008-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7325862
• 关键词: Air; Alloys; Analytical Chemistry; Biocompatible; Biological; Biological Markers; Bioterrorism; Chemicals; Complex; DNA; Detection; Development; Diagnosis; Diagnostic; Disease; Drug Design; Ensure; Fluorescence; Fluorescence Resonance Energy Transfer; Food; Genomics; Ligands; Malignant Neoplasms; Medical; Molecular Biology; Peptides; Phase; Protein Subunits; Proteins; Proteomics; Range; Research; Safety; Semiconductors; Series; Site; Small Business Funding Mechanisms; Small Business Innovation Research Grant; Spectrum Analysis; Surface; Techniques; United States National Institutes of Health; Water; absorption; base; improved; nanocrystal; pathogen; programs; quantum; structural biology; ultraviolet; vaccine development

DESCRIPTION (provided by applicant): Fluorescence-based multiplexed detection and spectroscopy is finding more and more applications in analytical chemistry, molecular biology, and medical diagnostics. For example, rapid advance in genomics and proteomics research has elucidated the availability of multiple biomarkers, of which simultaneous detection allow diagnosing the complex diseases such as cancer with significantly improved accuracy in comparison with the single marker detection. Rapid, sensitive and simultaneous detection of multiple pathogens in food, in water, and in air, is essential to ensure public safety and to protect the public against potential bioterrorism. In the modern structural biology, simultaneously probing the change in distances between different sites within a larger biomolecule such as multi-subunit proteins would provide valuable information that can be utilized for the development of rational drug design and rapid vaccine development for chemical and biological countermeasures. While, the availability of high quality fluoreagents with the high and distinguished fluorescent emission is the prerequisite for all above-mentioned applications. This NIH SBIR program will develop a series of alloyed tertiary semiconductor nanocrystals that have highly bright, stable, and narrow emission, are readily to be bio-conjugated with biomolecules such as proteins and DNA, and thus can be ideally be used as fluoreagents in multiplexed detection and spectroscopy.

描述（由申请人提供）：基于荧光的多路检测和光谱学在分析化学、分子生物学和医学诊断中得到越来越多的应用。例如，基因组学和蛋白质组学研究的快速发展已经阐明了多种生物标志物的可用性，其中同时检测可以诊断癌症等复杂疾病，与单一标记检测相比，其准确性显着提高。快速、灵敏和同时检测食物、水和空气中的多种病原体对于确保公共安全和保护公众免受潜在的生物恐怖主义至关重要。在现代结构生物学中，同时探测一个较大的生物分子（如多亚基蛋白）内不同位点之间距离的变化，将为合理的药物设计和快速的疫苗开发提供有价值的信息，用于化学和生物对策。而获得高质量的荧光剂是实现上述应用的前提条件。美国国立卫生研究院SBIR项目将开发一系列合金三级半导体纳米晶体，这些晶体具有高亮度，稳定和窄发射，易于与生物分子（如蛋白质和DNA）进行生物偶联，因此可以理想地用作多路检测和光谱中的荧光剂。