Novel 3-dimensional platform for high-throughput glycomics discovery

用于高通量糖组学发现的新型 3 维平台

• 批准号: 7218271
• 负责人: XICHUN ZHOU
• 金额: $ 10.18万
• 依托单位: ADA TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-02-01 至 2008-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7218271
• 关键词: 3-Dimensional; Address; Amines; Atomic Force Microscopy; Binding; Bioinformatics; Biological Process; Biomedical Research; Biopolymers; Carbohydrates; Cell Adhesion; Cell Differentiation process; Characteristics; Chemicals; Collaborations; Condition; Development; Event; Film; Glass; Glycoconjugates; Goals; Immobilization; Immune response; Laboratories; Left; Ligands; Marketing; Methods; Microarray Analysis; Microscope; Modeling; Modification; Monitor; Mono-S; Monosaccharides; Neoplasm Metastasis; Noise; Oligosaccharides; Oximes; Performance; Phase; Physical condensation; Polysaccharides; Population; Protein Binding; Protein-Carbohydrate Interaction; Proteins; Proteomics; Range; Research; Role; Signal Transduction; Site; Slide; Small Business Funding Mechanisms; Small Business Innovation Research Grant; Solid; Spottings; Structure; Surface; Surveys; Technology; Testing; Universities; Work; biochip; carbohydrate binding protein; carbohydrate receptor; carbonyl group; frontier; functional group; high throughput analysis; instrument; medical schools; microwave electromagnetic radiation; miniaturize; monolayer; neoplastic cell; novel; novel strategies; programs; sugar; tool; trafficking; two-dimensional

DESCRIPTION (provided by applicant): Glycomics is an emerging field that focuses on studying the interactions of carbohydrates with other biopolymers, particularly proteins, to gain a better understanding of the roles of carbohydrates in biological processes. Carbohydrate microarray technologies represent a new development at the frontier of glycomics. Microarrays of carbohydrates (glycoconjugates, polysaccharides, oligosaccharides and monosaccharides) allow high-throughput, parallel analyses. Parallel analyses greatly facilitate not only surveys of proteins for carbohydrate-binding activities but also elucidation of their ligands. However, there is a lack of technology for the fabrication of high quality carbohydrate microarrays for high-throughput analysis of glycomes because of the difficulty to reliably immobilize populations of chemically and structurally diverse glycans. ADA proposes to develop aminooxy- and hydrazide-functionalized dendrimer monolayer films on solid substrates for covalent, site-specific immobilization of unmodified carbohydrate probes. Miniaturized microwave radiation energy will also be evaluated for quick and convenient immobilization of the polysaccharides on the proposed slide platform. The resulting platforms will allow facile and effective fabrication of carbohydrate microarrays. The proposed novel slide platform will significantly increase the efficiency of glycan analysis and meet the growing needs of glycomics research and biomedical applications. Glycomics study the interactions of carbohydrates with other biopolymers, particularly proteins, to gain a better understanding of the roles of carbohydrates in biological processes. This proposed research seeks to develop a new tool for biologists to significantly increase the efficiency in glycan anaysis and meet the growing needs of glycomics research and biomedical applications.

描述(申请人提供)：糖链是一个新兴的领域，专注于研究碳水化合物与其他生物聚合物，特别是蛋白质的相互作用，以更好地了解碳水化合物在生物过程中的作用。碳水化合物微阵列技术代表了糖组学前沿的一项新发展。碳水化合物(糖共轭化合物、多糖、低聚糖和单糖)的微阵列可以进行高通量、平行分析。平行分析不仅极大地便利了对碳水化合物结合活性的蛋白质的调查，而且还有助于阐明它们的配体。然而，由于很难可靠地固定化化学和结构上不同的糖链群体，目前还缺乏用于高通量糖类分析的高质量碳水化合物微阵列的技术。ADA建议在固体基质上开发氨氧基和酰肼功能化的树枝状大分子单分子膜，用于共价、定点固定未修饰的碳水化合物探针。还将评估小型化的微波辐射能量，以快速和方便地将多糖固定在拟议的载玻片平台上。由此产生的平台将使碳水化合物微阵列的制造变得容易和有效。提出的新型玻片平台将显著提高多糖分析的效率，满足糖组学研究和生物医学应用的日益增长的需求。Glycomics研究碳水化合物与其他生物聚合物，特别是蛋白质的相互作用，以更好地了解碳水化合物在生物过程中的作用。这项拟议的研究旨在为生物学家开发一种新的工具，以显着提高多糖分析的效率，并满足糖组学研究和生物医学应用日益增长的需求。