Droplet based screening of molecular libraries in a microfluidic device

微流体装置中分子库的基于液滴的筛选

• 批准号: 7263950
• 负责人: DARREN R LINK
• 金额: $ 16.88万
• 依托单位: RAINDANCE TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-21 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7263950
• 关键词: Adsorption; Binding; Biocompatible Materials; Biological Assay; Boxing; Caliber; Cells; Charge; Chemicals; Cloning; Code; Collection; Complex; Computer Simulation; Development; Devices; Diffusion; Doctor of Philosophy; Drops; Drug Formulations; Dyes; Electrostatics; Elements; Encapsulated; Enzymes; Figs - dietary; Fluorescence; Fluorocarbons; Frequencies; Generations; Hour; Incubated; Individual; Libraries; Link; Liquid substance; Measures; Methods; Microfluidic Microchips; Microfluidics; Microspheres; Molecular Bank; Nucleic Acids; Oils; Operative Surgical Procedures; Phase; Polymerase Chain Reaction; Population; Process; Rate; Reaction; Reagent; Research Personnel; Screening procedure; Signal Transduction; Sorting - Cell Movement; Speed; Stream; Surface; System; Technology; Time; Week; aqueous; base; cytotoxicity; electric field; instrument; meter; particle; tool; wasting

DESCRIPTION (provided by applicant): The development of droplet-based microfluidic devices is driven by the need to screen large and varied libraries of molecules for interactions with populations of cells, enzymes, nucleic acids, and other biomolecular materials. Efficient screening requires an instrument to encapsulate individual elements of the molecular library with single or multiple cells, particles, or other biomolecules, perform an assay on the contents, perhaps through the addition of one or more reagents, and then, finally, to identify and selectively collect only the microreactors containing promising molecules. This requires a processing rate of 10A3 elements per second to sort through the smallest libraries in a reasonable time while rates on the order 10A5 per second are desirable for larger libraries. These rates are feasible by combining our proprietary microfluidic nanoreactor technology with high speed electric field based control and sorting. Upon successful completion of this integration, RainDance Technologies, Inc. will have developed an enabling technology capable of rapidly and economically screening enormous molecular libraries in timescale measured in hours instead of weeks.

描述（由申请人提供）：基于液滴的微流控装置的发展是由于需要筛选大量不同的分子文库，以便与细胞，酶，核酸和其他生物分子材料相互作用。有效的筛选需要一种仪器将分子文库的单个元素包裹在单个或多个细胞、颗粒或其他生物分子中，对其内容物进行分析，可能是通过添加一种或多种试剂，然后，最后，识别并选择性地收集含有有希望的分子的微反应器。这需要每秒10A3个元素的处理速率，以便在合理的时间内对最小的库进行排序，而对于较大的库，每秒10A5个元素的处理速率是理想的。通过将我们专有的微流控纳米反应器技术与基于高速电场的控制和分选相结合，这些速率是可行的。在成功完成此次整合后，RainDance Technologies, Inc.将开发出一种能够在数小时而不是数周的时间尺度上快速、经济地筛选大量分子库的技术。

项目成果

Reliable microfluidic on-chip incubation of droplets in delay-lines.

• DOI: 10.1039/b816049j
• 发表时间: 2009-05-21
• 期刊: Lab on a chip
• 影响因子: 6.1
• 作者: Frenz L;Blank K;Brouzes E;Griffiths AD
• 通讯作者: Griffiths AD