AB 4000 QTRAP Mass Spectrometer

描述(申请人提供)：小分子质谱学定量和定性分析，包括亲本、产物和中性损失扫描，以及在线性四极离子陷阱质谱仪中对翻译后蛋白质修饰，特别是磷酸化的高灵敏度表征，都是亚利桑那大学现有的NIH资助的研究人员推进其研究科学的迫切需要。应用生物系统公司需要为4000 Q陷阱质谱仪提供资金，以将这种混合的三重四极-线性离子陷阱添加到西南环境健康科学中心蛋白质组学核心和亚利桑那大学校园内的亚利桑那州癌症中心现有的质谱仪基础设施中。目前，已有10年历史的三重四极杆LC-MS/MS系统通过蛋白质组学核心用于一般服务，虽然它仍然具有功能和用途，但它受到许多项目的灵敏度要求的影响。亚利桑那大学校园里没有四极线型离子陷阱质谱仪系统。四极线性离子陷阱已被证明是迄今为止最好的蛋白质组谱分析和翻译后修饰图谱的质量分析器之一，因为它们增加了离子容量，提高了扫描速度，并具有实时“智能”扫描功能。该仪器的收购得到了来自药理学和毒理学、分子和细胞生物学、化学、病理学、生化和分子生物物理学、细胞生物学和解剖学以及植物科学系的11名NIH资助的研究人员的支持，他们都曾有过MS的经验和服务。使用该仪器的战略重点领域将是(1)小分子定量/表征和(2)多肽磷酸化鉴定/表征。提供了制造商的示范数据，并与现有的来自这些用户的真实样品的质谱学资源进行了比较分析，清楚地展示了这种新的最先进的质谱仪的必要性和实用性。亚利桑那大学在经验丰富的人员、组织和管理能力以及使用记录方面的支持系统已经存在，这使得收购4000 Q陷阱成为应对未来基于MS的研究和科学挑战的合乎逻辑的选择。

DESCRIPTION (provided by applicant): Small molecule mass spectrometric quantitative and qualitative analyses including parent, product and neutral loss scans, as well as highly sensitive characterization of post translational protein modifications, specifically phosphorylation, in a linear quadrupole ion trap mass spectrometer, are both highly needed capabilities for existing NIH-funded investigators at the University of Arizona to advance the science of their research. Funds for a 4000 Q TRAP mass spectrometer from Applied Biosystems are requested to add this hybrid triple quadrupole-linear ion trap to the existing mass spectrometry instrumentation infrastructure of the Proteomics Core of the Southwest Environmental Heath Science Center and the Arizona Cancer within the University of Arizona campus. Currently one 10-yr old triple quadrupole LC-MS/MS system is available through the Proteomics Core for general service use and while it is still functional and useful, it suffers from the sensitivity requirements of many projects. No quadrupole linear ion trap MS system is available on the University of Arizona campus. Quadrupole linear ion traps have been shown to be among the best mass analyzers to date for highly sensitive proteome profiling and for post translational modification mapping due to their increased ion capacity, increased scanning speed, and real-time "smart" scanning functions. Acquisition of this instrument is being supported by 11 NIH-funded investigators from the Departments of Pharmacology and Toxicology, Molecular and Cellular Biology, Chemistry, Pathology, Biochemical and Molecular Biophysics, Cell Biology and Anatomy, and Plant Sciences, all of whom have had MS experience and services performed in the past. The strategic areas of focus for use of this instrument will be (1) small molecule quantitation/characterization and (2) peptide phosphorylation identification/characterization. Manufacturer demonstration data and comparative analyses with existing mass spectrometry resources of realistic samples from these users are presented, and clearly showcase the need and utility for this new state-of-the art mass spectrometer. The support system at the University of Arizona in terms of experienced personnel, organizational and management abilities and usage track record, exists, making acquisition of the 4000 Q TRAP a logical choice to handle the MS-based research and scientific challenges of the future.