AB 4000 QTRAP MASS SPECTROMETER: CHEMISTRY

AB 4000 QTRAP 质谱仪：化学

• 批准号: 7335129
• 负责人: George Tsaprailis
• 金额: $ 3.57万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335129
• 关键词: experience; ions; mass spectrometry; phosphorylation; proteomics; small molecule; university

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): Small molecule mass spectrometric quantitative and qualitative analyses including parent, product and neutral loss scans, as well as highly sensitive characterization of post translational protein modifications, specifically phosphorylation, in a linear quadrupole ion trap mass spectrometer, are both highly needed capabilities for existing NIH-funded investigators at the University of Arizona to advance the science of their research. Funds for a 4000 Q TRAP mass spectrometer from Applied Biosystems are requested to add this hybrid triple quadrupole-linear ion trap to the existing mass spectrometry instrumentation infrastructure of the Proteomics Core of the Southwest Environmental Heath Science Center and the Arizona Cancer within the University of Arizona campus. Currently one 10-yr old triple quadrupole LC-MS/MS system is available through the Proteomics Core for general service use and while it is still functional and useful, it suffers from the sensitivity requirements of many projects. No quadrupole linear ion trap MS system is available on the University of Arizona campus. Quadrupole linear ion traps have been shown to be among the best mass analyzers to date for highly sensitive proteome profiling and for post translational modification mapping due to their increased ion capacity, increased scanning speed, and real-time "smart" scanning functions. Acquisition of this instrument is being supported by 11 NIH-funded investigators from the Departments of Pharmacology and Toxicology, Molecular and Cellular Biology, Chemistry, Pathology, Biochemical and Molecular Biophysics, Cell Biology and Anatomy, and Plant Sciences, all of whom have had MS experience and services performed in the past. The strategic areas of focus for use of this instrument will be (1) small molecule quantitation/characterization and (2) peptide phosphorylation identification/characterization. Manufacturer demonstration data and comparative analyses with existing mass spectrometry resources of realistic samples from these users are presented, and clearly showcase the need and utility for this new state-of-the art mass spectrometer. The support system at the University of Arizona in terms of experienced personnel, organizational and management abilities and usage track record, exists, making acquisition of the 4000 Q TRAP a logical choice to handle the MS-based research and scientific challenges of the future.

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：小分子质谱定量和定性分析，包括母体、产物和中性丢失扫描，以及线性四极离子阱质谱仪中翻译后蛋白质修饰（特别是磷酸化）的高灵敏度表征，都是亚利桑那大学现有NIH资助的研究人员迫切需要的能力，以推进他们的研究科学。应用生物系统公司的4000 Q TRAP质谱仪的资金被要求将这种混合三重四极线性离子阱添加到西南环境健康科学中心和亚利桑那大学校园内的亚利桑那癌症的蛋白质组学核心的现有质谱仪器基础设施中。目前，一个10年的三重四极杆LC-MS/MS系统可通过蛋白质组学核心用于一般服务使用，虽然它仍然是功能和有用的，它受到许多项目的灵敏度要求。亚利桑那大学校园内没有四极杆线性离子阱MS系统。四极线性离子阱已被证明是迄今为止最好的质量分析仪之一，用于高灵敏度蛋白质组分析和翻译后修饰作图，这是由于其增加的离子容量，增加的扫描速度和实时“智能”扫描功能。该仪器的获得得到了来自药理学和毒理学，分子和细胞生物学，化学，病理学，生物化学和分子生物物理学，细胞生物学和解剖学以及植物科学部门的11名NIH资助的研究人员的支持，他们都有MS的经验和服务。使用该仪器的战略重点领域将是（1）小分子定量/表征和（2）肽磷酸化鉴定/表征。制造商演示数据和比较分析与现有的质谱资源的现实样品从这些用户，并清楚地展示了这种新的国家的最先进的质谱仪的需要和实用性。亚利桑那大学在经验丰富的人员，组织和管理能力以及使用记录方面的支持系统存在，使得收购4000 Q TRAP成为处理基于MS的研究和未来科学挑战的合理选择。