A Biomedical Mass Spectrometry Resource: Ongoing Driving Biomedical Projects

生物医学质谱资源：持续推动生物医学项目

• 批准号: 10441142
• 负责人: MICHAEL L GROSS
• 金额: $ 65.99万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10441142
• 关键词: Affinity; Alzheimer' s Disease; Amplifiers; Amyloid beta-Protein; Antibodies; Area; Automobile Driving; Back; Binding; Binding Proteins; Biochemistry; Biological; Biology; Biomedical Research; Calcium; Cardiolipins; Ceramides; Chemistry; Collaborations; Communities; Complex; Computer Interface; Cryoelectron Microscopy; Dengue; Dengue Fever; Development; Diabetes Mellitus; Disease; Ebola; Education and Outreach; Epitopes; Fourier transform ion cyclotron resonance; Funding; Future; Goals; Government; Grant; Guidelines; Health; Human; Industrialization; International; Investigation; Investments; Ions; Isotopes; Laboratories; Lasers; Leadership; Leishmania; Light; Lipids; Location; Maintenance; Malaria; Malignant Neoplasms; Maps; Marburgvirus; Mass Spectrum Analysis; Medicine; Membrane Proteins; Metabolic; Metabolic syndrome; Metabolism; Metals; National Institute of General Medical Sciences; Parkinson Disease; Pathogenesis; Pathogenicity; Phospholipids; Physiologic pulse; Plasmodium; Positioning Attribute; Property; Protein Footprinting; Proteins; Proteomics; Recording of previous events; Regulation; Research; Research Personnel; Research Support; Resources; Role; Scientist; Services; Source; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sphingolipids; Structure; Triglycerides; Tuberculosis; Universities; Urinary tract infection; Virus Diseases; Washington; Work; ZIKA; acylcarnitine; antigen binding; biomedical resource; experience; human disease; innovation; instrument; instrumentation; lipid metabolism; lipidomics; mass spectrometer; medical schools; metabolomics; microorganism; novel strategies; nutrition; pathogenic bacteria; pathogenic virus; phosphoneuroprotein 14; preservation; programs; protein aggregation; protein complex; protein folding; proteogenomics; skills; tandem mass spectrometry; tau Proteins; technology research and development; tool; user-friendly

Project Summary The Washington University NIGMS Biomedical Resource seeks to continue its successful collaborative work with biomedical researchers engaged in understanding disease and maintaining human health. This Resource supports a wide array of scientists requiring structural proteomics and complex lipidomics by expertly using mass spectrometry tools, many of which were developed at this Resource over the last 15 years. The dedicated scientists at the Resource have more than a century of combined experience and continue to collaborate with investigators working on important biomedical problems including viral pathogens like Ebola, Dengue, Zika, and Marburg, bacterial pathogens such as the causative agents of tuberculosis and leishmania, plasmodia that cause malaria, Alzheimer's and Parkinson's diseases, diabetes, metabolic syndrome, urinary tract infections, and cancer. Resource staff investigators, motivated by national and international collaborators in academic, industrial, and governmental laboratories, provide support for the study of cellular calcium regulation, lipid metabolism, antibody-antigen binding, and many other problems in structural and functional biology. In addition, the dedicated scientists at this Resource will support successful dissemination, training, and outreach to the scientific and broader communities. This grant will allow them to continue the application of mass spectrometry to these problems as they transition to other sources of support that allow them to honor commitments to their large and diverse group of collaborators.

项目概要 华盛顿大学 NIGMS 生物医学资源寻求继续其成功的合作工作 与致力于了解疾病和维护人类健康的生物医学研究人员合作。 This Resource 通过熟练地使用，支持需要结构蛋白质组学和复杂脂质组学的广泛科学家 质谱工具，其中许多工具是在过去 15 年中在该资源中开发的。这 该资源的敬业科学家拥有一个多世纪的综合经验，并将继续 与研究重要生物医学问题的研究人员合作，包括埃博拉等病毒病原体， 登革热、寨卡病毒和马尔堡病毒，细菌病原体，例如结核病和利什曼原虫的病原体， 导致疟疾、阿尔茨海默病和帕金森病、糖尿病、代谢综合征、泌尿系统疾病的疟原虫 tract infections, and cancer.在国内和国际合作者的推动下，资源人员调查员 在学术、工业和政府实验室，为细胞钙的研究提供支持 调节、脂质代谢、抗体-抗原结合以及许多其他结构和功能问题 生物学。此外，该资源的敬业科学家将支持成功的传播、培训、 并向科学界和更广泛的社区进行推广。这笔赠款将使他们能够继续申请 当他们转向其他支持来源时，质谱仪可以解决这些问题，使他们能够兑现 对庞大且多样化的合作者群体的承诺。

项目成果

Development of a High-Throughput Mass Spectrometry-Based SARS-CoV-2 Immunoassay.

• DOI: 10.1021/acs.analchem.3c02421
• 发表时间: 2023-12
• 期刊: Analytical chemistry
• 影响因子: 7.4
• 作者: Jie Sun;Jong Hee Song;Mary K Danielson;Nathan D Colley;Alia Thomas;David Hambly;J. C. Barnes;Michael L. Gross