Olympus FV 1000 Confocal Microscope for Neurobiology and Cellular Biology Researc

描述（由申请人提供）：本申请申请资金购买一台Olympus Fluoview (FV) 1000光谱单元激光扫描共聚焦显微镜，供美国国立卫生研究院资助的一个互动研究小组使用，主要位于赖特州立大学解剖和生理学系。该单元还可与分布在医学院其他几个系和科学与数学学院的其他研究人员共享使用。这种新的共聚焦系统将有两个主要目的，1)取代我们已有6年历史的奥林巴斯FX， 2)升级我们的共聚焦仪器功能。新系统提供的主要进展包括a)超越我们目前的两个通道限制的多重荧光标记；B)激光照明的最优控制；c)改进了荧光染料的分离，即使在发射曲线密切相关的情况下也是如此；d)改进了文件处理、保存和导出，以便在2D和3D空间中进一步进行定量分析。该仪器将主要由五名研究人员组成的核心小组使用，他们将形成一个合作单位，在发育、损伤和损伤恢复过程中对突触和电路的修改有共同的项目和共同的科学兴趣。他们还积累了广泛的成像设备和定量方法的专业知识，其中许多需要改进的奥林巴斯FV 1000系统的能力。进一步用户的研究主题多种多样，从神经元的体积或pH控制到细胞钙运输机制和红细胞和神经元中的氯化物稳态，再到中枢神经系统对呼吸功能的控制。大多数研究人员（主要或次要用户）由NIH资助。为了促进维护和公平使用，该仪器将被纳入脑研究中心的显微镜和成像设施。这个核心设施成立于2000年，其使命是为主要（但不限于）医学院的研究人员提供成像和组织学需求（电子显微镜，图像分析，神经元追踪，共聚焦显微镜，显微切开术和超显微切开术），重点是神经科学研究。因此，收购奥林巴斯FV 1000系统将大大加强核心研究人员的研究计划，但它也将对赖特州立大学更广泛的研究社区产生有益的影响。

DESCRIPTION (provided by applicant): This proposal requests funds to purchase an Olympus Fluoview (FV) 1000 Spectral Unit Laser Scanning Confocal Microscope to be utilized by an interactive group of NIH-funded investigators, primarily located in the Department of Anatomy and Physiology at Wright State University. The unit will be also available for shared use with other investigators distributed throughout several other Departments of the Medical School and the College of Science and Mathematics. This new confocal system will serve two major purposes, 1) replacement of our six year-old Olympus FX and 2) upgrade our confocal instrumentation capabilities. Major advances provided by the new system include a) multiple fluorochrome labeling beyond our current two channel limitations; b) optimal controls of laser illumination; c) improved fluorochromes separation, even when emission curves are closely related and d) improved file handling, save and export for further quantitative analyses in 2D and 3D space. The instrument will be mostly used by a core group of five investigators that will form a collaborative unit with shared projects and common scientific interests on the modification of synapses and circuits during development, injury and recovery from injury. They have also accumulated a broad range of expertise with imaging equipment and quantitative methods, many of which require the improved capabilities of the Olympus FV 1000 system. The research topics from further users are varied and range from volume or pH control in neurons to cellular calcium transport mechanisms and chloride homeostasis in red blood cells and neurons to central nervous system control of respiratory function. The majority of investigators (major or minor users) are funded through NIH. To promote maintenance and equitable use, the instrument will be integrated within the microscopy and imaging facilities of the Center for Brain Research. This core facility was established in 2000 with the mission of providing imaging and histological needs (electron microscopy, image analysis, neuron tracing, confocal microscopy, microtomy and ultramicrotomy) to researchers located primarily (but not exclusively) in the School of Medicine and with an emphasis in Neuroscience research. As such, the acquisition of the Olympus FV 1000 systems will significantly enhance the research programs of the core investigators, but it will also have a beneficial impact on the broader research community at Wright State University.