Indiana University Bloomington (IUB) Center for Cannabis, Cannabinoids, and Addiction (C3A)

摘要 我们建议建立布卢明顿大麻、大麻和成瘾中心(IUB-C3A) 作为NIDA卓越核心中心，为中西部中部和整个地区的成瘾研究人员提供服务 这个国家。该中心将提供核心服务，以加深我们对基本大脑过程的理解 导致或之后使用成瘾药物，尤其是大麻。IUB-C3A将由两个 服务核心、试点项目核心和行政核心。行政核心将提供一个良好的- 为有效的中心管理、公共宣传、组织中心的课程确定的结构，如 以及保存和方便地获取中心科学家产生的数据。这个 生物活性脂质介体核心(BLMC)将为检测大麻素和其他 生物样本中的生物活性脂质介体，并针对服务不足的少数群体举办夏季课程 对STEM职业感兴趣的大学生。多尺度成像核心(MSIC)将提供服务和 通过一系列光学显微镜成像方式进行培训。MSIC的多光子资源将 包括培训和获得钙和其他传感器(例如，神经调节剂)体内纵向成像 例如内源性大麻素、多巴胺和5-羟色胺)以及远程途径 在“清除”的大脑样本中进行追踪。MSIC的STORM/COMPUS资源将为用户提供 有机会进行从宏观到纳米级别的相关结构/功能研究，还将 提供有关这些技术的课程。两个核心都将强调各自的创新和整合 技术，如提案中所概述的。试点项目核心将向C3A附属公司和 药物滥用领域以外的调查人员并指导他们完成获取数据和NIDA的过程 支持他们的研究想法。IUB-C3A的PI有着长期的富有成效的合作历史， 包括一起发表了60多篇论文，并获得了多项MPI NIH拨款。IUB-C3A是 被概念化为一种资源，将为中环各地的其他成瘾调查人员提供机会 中西部(伊利诺伊州南部、印第安纳州、肯塔基州、俄亥俄州和西弗吉尼亚州)，这是一个深受毒品影响的地区 上瘾，而且是在全国范围内。Iub-C3A旨在通过一项 夏季经验与针对代表不足人群的试点项目方案相结合 神经科学和毒瘾。这些计划的一个核心目标是将有才华的人带入 上瘾研究。我们预计他们将采用创造性的指导，严格的实验方法， 以及应对重大公共卫生问题的新颖思维方式。印第安纳大学对此给予大力支持 在设备采购和我们的多元化计划上，慷慨的配套资金证明了我们的努力。全 在这些因素中，预测IUB-C3A将成为区域和国家更好地了解的资源 以及开发成瘾障碍及其后果的治疗方法。

Abstract We are proposing to establish the IU Bloomington Center for Cannabis, Cannabinoid, and Addiction (IUB-C3A) as a NIDA Core Center for Excellence to serve addiction researchers both in the central Midwest and across the nation. This Center will offer core services to further our understanding of fundamental brain processes leading to or following the use of addictive drugs, particularly cannabis. The IUB-C3A will consist of two service cores, a pilot project core, and an administrative core. The Administrative Core will provide a well- defined structure for efficient center management, for public outreach, for organizing the Center’s courses, as well as for preserving and making easily accessible the data generated by the Center’s scientists. The Bioactive Lipid Mediators Core (BLMC) will provide analytical service for detecting cannabinoids and other bioactive lipid mediators in biological samples and run a summer course directed towards underserved minority college students interested in STEM careers. The MultiScale Imaging Core (MSIC) will offer services and training across a range of light microscopic imaging modalities. The multiphoton resource of the MSIC will include training and access to longitudinal in vivo imaging of calcium and other sensors (e.g., neuromodulators such as endocannabinoids, dopamine, and serotonin) from very young ages as well as long-range pathway tracing in “cleared” brain specimens. The STORM/confocal resource of the MSIC will offer users the opportunity to perform correlative structure/function studies from the macro- to nanoscale level and will also offer courses on these techniques. Both cores will emphasize innovation and integration of their respective techniques, as outlined in the proposal. The Pilot Project Core will solicit pilot projects from C3A Affiliates and investigators outside of the drug abuse field and mentor them through the process of obtaining data and NIDA support for their research ideas. The PIs for the IUB-C3A have a long history of productive collaborations, including publishing more than sixty papers together and holding several MPI NIH grants. The IUB-C3A is conceptualized as a resource that will offer opportunities for other addiction investigators across the Central Midwest (Southern Illinois, Indiana, Kentucky, Ohio, and West Virginia), a region strongly affected by drug addiction, and across the nation. The IUB-C3A aims to increase diversity in addiction research through a combination of summer experiences and pilot project programs targeted to under-represented populations in neuroscience and addiction. A core goal of these programs is to bring talented individuals into the field of addiction research. We anticipate that they will apply creative directions, rigorous experimental approaches, and novel ways of thinking to a major public health problem. The strong support of Indiana University to this endeavor is evident by generous matching funds for both equipment purchases and our diversity programs. All of these factors predict that the IUB-C3A will become a regional and national resource for better understanding and developing treatments for addictive disorders and their consequences.