CCR RNAi Initiative: Establishment of shRNA RNAi Library

CCR RNAi 倡议：shRNA RNAi 文库的建立

• 批准号: 7338745
• 负责人: natasha caplen
• 金额: --
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7338745
• 关键词: CCR; RNAi; Initiative; Establishment; shRNA

In 2006 the Gene Silencing Section, Genetics Branch took delivery of a whole human genome pre-constructed shRNA library (60,000 clones) initially generated by Drs. G. Hannon (Cold Spring Harbor Laboratory) and S. Elledge (Harvard Unviversity). In collaboration with Dr. Paul Meltzer's group (Genetics Branch, CCR) three complete replicas of this library have now been grown and quality control analysis will be initiated in FY07. We have enabled a limited number of CCR investigators to access clones from this library and we plan to continue this in the future. Once we have conducted key quality control studies such as sequencing, transfection optimization, and baseline RNAi analysis we have a number of independent and collaborative studies that we plan to initiate.

2006年，基因沉默科遗传学分支接收了由G. Hannon（冷泉港实验室）和S. Elledge（哈佛大学）。与Paul Meltzer博士的小组（CCR遗传学分支）合作，现已培养出该文库的三个完整复制品，并将于2007财年启动质量控制分析。我们已经使有限数量的CCR研究人员能够从这个文库中访问克隆，我们计划在未来继续这样做。一旦我们进行了关键的质量控制研究，如测序，转染优化和基线RNAi分析，我们有一些独立的和合作的研究，我们计划启动。