RNAi analysis of the ATP-binding cassette (ABC) family o

ATP 结合盒 (ABC) 家族的 RNAi 分析

• 批准号: 7292884
• 负责人: natasha caplen
• 金额: --
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7292884
• 关键词: RNAi; analysis; ATP; binding; cassette

Extensive associative gene expression data has been developed showing links between the expression of key members of the ABC family of proteins and resistance to many of the most commonly used anti-cancer chemotherapeutic agents. Current methods to analyze the direct functional consequence of the expression of the multiple drug resistance (MDR) proteins has been hampered by the lack of specificity of drug inhibitors, and the probable overlapping functional effect of these proteins when tumor cells develop MDR. The Laboratory of Cell Biology, CCR has recently identified a compound that appears to specifically be cytotoxic to cells over expressing MDR-1 (ABC-B1). Using fully characterized siRNAs corresponding to ABC-B1 we have now shown that MDR-1 expression does modulate the sensitivity of cells to this compound. We are continuing to collaborate with LCB to further understand the mechanism of action of this and related drugs. This data was presented as part of an Oral presentation at the 2005 AACR meeting and is included within a manuscript recently submitted for publication.

广泛的关联基因表达数据已被开发，显示ABC家族蛋白质的关键成员的表达与对许多最常用的抗癌化疗剂的抗性之间的联系。目前分析多药耐药（MDR）蛋白表达的直接功能后果的方法受到药物抑制剂缺乏特异性以及当肿瘤细胞发生MDR时这些蛋白可能重叠的功能作用的阻碍。CCR细胞生物学实验室最近发现了一种化合物，该化合物似乎对过表达MDR-1（ABC-B1）的细胞具有特异性细胞毒性。使用完全表征的对应于ABC-B1的siRNA，我们现在已经表明MDR-1表达确实调节细胞对该化合物的敏感性。我们正在继续与LCB合作，以进一步了解这种药物和相关药物的作用机制。这些数据是作为2005年AACR会议口头报告的一部分提出的，并包括在最近提交出版的手稿中。