CCR RNAi Initiative: Establishment of shRNA RNAi Library Screens

CCR RNAi 计划：shRNA RNAi 文库筛选的建立

• 批准号: 7592800
• 负责人: natasha caplen
• 金额: $ 20.95万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7592800
• 关键词: Bacteria; CCR; Cell physiology; Collaborations; Complement; Development; Gene Silencing; Genes; Genetic; Genome; Goals; Harvest; Human; Human Genome; Laboratories; Libraries; Mediating; Numbers; Pharmaceutical Preparations; Protocols documentation; Quality Control; RNA Interference; RNA library; Research; Resources; Small Interfering RNA; Universities; gene function; plasmid DNA; small hairpin RNA

In 2006 the Gene Silencing Section, Genetics Branch took delivery of a whole human genome pre-constructed short hairpin RNA (shRNA) library (60,000 clones) initially generated by Drs. G. Hannon (Cold Spring Harbor Laboratory) and S. Elledge (Harvard University). In collaboration with Dr. Paul Meltzers group (Genetics Branch, CCR) three complete replicas of this library have now been grown and quality control analysis has been initiated, including determination of high-throughput protocols for the growing and harvesting of bacteria harboring shRNAs and purification of high quality plasmid DNA. To date we have characterized the silencing mediated by multiple shRNAs corresponding to only a limited number of human genes but envisage that this number will increase rapidly.

2006年，基因沉默组，遗传学分支接收了一个完整的人类 基因组预构建的短发夹RNA（shRNA）文库（60，000个克隆）最初产生 G博士Hannon（冷泉港实验室）和S. Elledge（哈佛大学）。在 与Paul Meltzers博士团队（CCR遗传学分支）合作完成三项 该文库的复制品现在已经生长并且质量控制分析已经开始， 包括确定用于生长和收获 携带shRNA的细菌和高质量质粒DNA的纯化。迄今为止， 其特征在于由仅对应于有限数量的多个shRNA介导的沉默 但预计这一数字将迅速增加。