RNAi for the identification of Hypoxia responsive genes

RNAi 用于鉴定缺氧反应基因

• 批准号: 7292899
• 负责人: natasha caplen
• 金额: --
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7292899
• 关键词: RNAi; identification; Hypoxia; responsive; genes

A project has been recently initiated to conduct a systematic analysis of hypoxia responsive genes using RNAi. Several investigators are interested in examining the hypoxic response of cells. The biologic stimulus of anoxia could be introduced into cell lines including HeLa, 7860 Renal Cell CA or DU-145 (with or without drug intervention) following shRNA introduction as a means of dissecting components of the hypoxia pathway. To date we have analyzed siRNAs against the following hypoxia associated genes -HIF-1A, HIF1An-1 and HIF3A. Follow up validation and phenotypic analysis has begun. In addition, we have purchased through Open Biosystems shRNA clones corresponding to HIF1A, HIF2A, ARNT, HSPCA and VEGF). Following validation at an RNA level we will be in a position to both assess the efficiency with which shRNA clones from the CSHL are working and will be able to use this for small-scale functional analysis.

最近启动了一个项目，利用 RNAi 对缺氧反应基因进行系统分析。一些研究人员对检查细胞的缺氧反应感兴趣。在引入 shRNA 后，可以将缺氧的生物刺激引入细胞系，包括 HeLa、7860 肾细胞 CA 或 DU-145（有或没有药物干预），作为剖析缺氧途径成分的手段。迄今为止，我们已经分析了针对以下缺氧相关基因 -HIF-1A、HIF1An-1 和 HIF3A 的 siRNA。后续验证和表型分析已经开始。此外，我们还通过 Open Biosystems 购买了对应于 HIF1A、HIF2A、ARNT、HSPCA 和 VEGF 的 shRNA 克隆。在 RNA 水平上进行验证后，我们将能够评估 CSHL 的 shRNA 克隆的工作效率，并能够将其用于小规模功能分析。