Clinical Nutrition Research Unit

临床营养研究室

• 批准号: 7333089
• 负责人: MICHAEL SCHWARTZ
• 金额: $ 16.6万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7333089
• 关键词: Address; Adipose tissue; Animal Experimentation; Animal Housing; Animals; Area; Arterial Lines; Arts; Biomedical Research; Biopsy Specimen; Body Composition; Body Temperature; Bone Marrow Transplantation; Breeding; Budgets; Carbohydrates; Cardiac; Chronic; Clinical Nutrition; Collaborations; Commit; Daily; Diabetes Mellitus; Diabetic Angiopathies; Dietary Intervention; Disease; Educational process of instructing; Endocrinology; Energy Metabolism; Ensure; Environmental Health; Equipment; Evolution; Faculty; Fee-for-Service Plans; Floor; Food; Funding; Future; Genes; Genetic; Genotype; Glucose Clamp; Goals; Growth; Health Services Accessibility; Housing; IACUC; Indirect Calorimetry; Interdisciplinary Study; Joints; Laboratories; Letters; Lipids; Measures; Medicine; Messenger RNA; Metabolic; Modeling; Molecular Biology; Monitor; Morphology; Motor Activity; Mus; Numbers; Nutrient; Nutritional Study; Obesity; Operative Surgical Procedures; Organizational Change; Pattern; Phenotype; Physical activity; Physiology; Polymerase Chain Reaction; Preparation; Procedures; Process; Proteins; Protocols documentation; Quality Control; Rate; Rattus; Research; Research Personnel; Resources; Risk; Rodent; Rodent Model; Schools; Services; Techniques; Testing; Thermogenesis; Time; Tissue Sample; Tissues; Transgenic Mice; Treatment Protocols; Triglycerides; Universities; Update; Venous; Washington; Water consumption; base; comparative; energy balance; experience; genetic analysis; implantation; in vivo; insulin secretion; islet; medical schools; mutant; new technology; nutrition; organizational structure; programs; research facility; response; size; success

ANIMAL STUDIES CORE I. OVERVIEW OF CHANGES TO THE CORE During the current funding cycle, the Animal Studies Core has been revised with respect both to its organizational structure and to the scope and sophistication of services offered to Affiliate Investigators. These changes have been made possible, in part, through a major increase of institutional commitment in support of the Core's various functions and due to the changing needs of Affiliate Investigators. The following summary highlights these changes: ¿ Organizational changes. Prior to this competing renewal, the CNRU supported basic animal research through two distinct Core laboratories - the Rodent Energy Metabolism and Body Composition Core (Dr. Michael Schwartz, Director) and the Nutrient Gene Core (Dr. Renee LeBoeuf, Director). To better meet the needs of Affiliate Investigators (and in response to input from our External Advisory Board), these two Core laboratories are now merged into a single entity termed the "Animal Studies Core", with Dr. Schwartz serving as Director, Dr. LeBoeuf as Co-Director, and Dr. Gregory Morton as Associate Director. ¿ Institutional commitment. To meet expanding demand for Core services, and in conjunction with the implementation of the new NIH-funded Mouse Metabolic Phenotyping Center (MMPC), the University of Washington School of Medicine has committed new, state-of-the-art research space and animal housing space within its newest biomedical research facility located at the South Lake Union (SLU) campus in downtown Seattle. Institutional funds have also been committed by UW in support of this program, as summarized below: ¿ Research space. Included is an approximate doubling of space for metabolic cage studies, expanding this resource from its current size (8 mouse and 4 rat cages; total of 12 cages) to accommodate 16 cages for rats and 16 for mice (total of 32 cages). New wet lab space will also be made available both for surgical procedures (e.g., implantation of body temperature monitor and chronic indwelling venous and arterial line insertion) and in vivo study procedures (e.g., glucose clamp studies), reflecting an overall marked expansion of research space committed to the Animal Studies Core. (Equipment funds are requested to support this expansion; see Animal Studies Core Budget.) ¿ Adjacent animal housing space. In addition to wet lab space, new animal housing quarters will be installed adjacent to the Animal Studies Core laboratory, allowing study animals received from Affiliate Investigators to be boarded until such time as studies are performed, averting the potential risk to animals located in the regular animal housing area, one floor below the Core laboratory. ¿ Creation of a new UW Obesity/Diabetes Center of Excellence, based at the SLU campus. A key priority of this Center will be to meet current and future animal research needs of investigators involved in obesity research. Additional priorities include support for junior investigators, additional pilot and feasibility funding, and promotion of interdisciplinary research across programs, departments and schools both within and outside of UW. ¿ Transfer of some services to the Analytic Core. During previous funding cycles, the Nutrient Gene Core participated in limited numbers of microassays of proteins, lipids, and carbohydrates, which now has largely been taken over by the Analytic Core. However, staff of the Genetics Component of the Animal Studies Core will continue to teach and advise with respect to quantification of mRNA species, thereby enabling Affiliate Investigators to set up appropriate tests in their own laboratory. Techniques include mRNA isolation from adipose and other tissues. Services offered directly by the Animal Studies Core are: Physiology Component (Dr. Morton, Associate Director): ¿ indirect calorimetry (e.g., metabolic rate, heat production) ¿ locomotor activity/total physical activity ¿ meal pattern analysis/continuous measures of total daily food and water intake ¿ continuous body temperature monitoring ¿ noninvasive body composition analysis ¿ biopsy sample triglyceride content ¿ euglycemic glucose clamp Genetics Component (Dr. LeBoeuf, Director): ¿ genotyping of mutant models ¿ breeding strategy for mouse colonies ¿ quantitative PCR of mRNA in tissue samples ¿ Meal preparation for nutritional interventions ¿ Bone marrow transplantation procedures Services offered indirectly via affiliated cores, with the Animal Studies Core serving as a conduit are: ¿ Diabetes complications (microvascular, macrovascular, cardiac) ¿ Molecular biology (gene sequencing, riboprobe preparation) ¿ Insulin secretion/islet morphology ¿ Creation of transgenic mice These services will be performed on a fee-for-service basis by the Diabetes Endocrinology Research Center (DERC), the new Mouse Metabolic Phenotyping Center (MMPC), and the Center for Ecogenetics and Environmental Health (CEEH). Arrangements have been made with these Centers (see letters of collaboration) for CNRU Affiliate Investigators to have access to the services noted above.

动物研究核心 I.核心变更概述 在当前的资助周期内，动物研究核心已经在其两个方面进行了修订。 组织结构以及向关联研究者提供的服务的范围和复杂性。这些 改革之所以成为可能，部分原因是机构承诺大幅增加， 核心研究者的各种职能以及附属研究者不断变化的需求。以下概述 强调了这些变化： 组织变革。在这次竞争性更新之前，CNRU支持基础动物研究， 通过两个不同的核心实验室-啮齿动物能量代谢和身体成分核心（博士。 Michael Schwartz，Director）和Nutrient Gene Core（Dr. Renee LeBoeuf，Director）。更好地满足 为了满足附属研究者的需求（并响应我们外部顾问委员会的意见），这两个核心 实验室现在合并成一个单一的实体，称为“动物研究核心”，与施瓦茨博士 LeBoeuf博士为联合主任，Gregory Morton博士为副主任。 机构承诺。为满足对核心服务不断增长的需求，并配合 实施新的NIH资助的小鼠代谢表型中心（MMPC），大学 华盛顿医学院已承诺新的，国家的最先进的研究空间和动物住房 位于南湖联盟（SLU）校园的最新生物医学研究设施内的空间， 西雅图市中心。机构资金也已承诺由华盛顿大学支持这一计划，因为 摘要如下： 研究空间。包括代谢笼研究的空间大约增加了一倍， 将这一资源从目前的规模（8个小鼠笼和4个大鼠笼;总共12个笼）扩大到 容纳16个大鼠笼和16个小鼠笼（共32个笼）。新的湿实验室空间还将 可用于外科手术（例如，体温监测器植入 和慢性留置静脉和动脉管线插入）和体内研究程序（例如， 葡萄糖钳夹研究），反映了致力于 动物研究中心（需要设备资金来支持这一扩张;见动物 核心预算） 临近动物居住区。除了潮湿的实验室空间，新的动物宿舍将 安装在动物研究中心实验室附近，允许接收研究动物 从附属研究者登机，直到研究进行，避免 对位于核心下方一层的常规动物饲养区的动物存在潜在风险 实验室 创建一个新的UW肥胖/糖尿病卓越中心，总部设在SLU校园。一 该中心的主要优先事项将是满足当前和未来的动物研究需求， 参与肥胖症研究的研究人员。其他优先事项包括支持初级 研究人员、额外的试点和可行性资金以及促进跨学科研究 跨方案，部门和学校内外的UW。 将一些服务转移到分析核心。在以往的供资周期中， Gene Core参与了有限数量的蛋白质、脂质和 碳水化合物，现在已在很大程度上被分析核心接管。然而，工作人员 动物研究核心的遗传学部分将继续教授和建议， 关于mRNA种类的定量，从而使附属研究者能够建立 在自己的实验室进行适当的测试。技术包括从脂肪组织中分离mRNA， 其他组织。 动物研究中心直接提供的服务包括： 生理学组件（Morton博士，副总监）： 间接量热法（例如，代谢率，产热） 自主活动/总体力活动 膳食模式分析/每日总食物和水摄入量的连续测量 连续体温监测 无创人体成分分析 活检样本甘油三酯含量 正葡萄糖钳夹 遗传学部分（LeBoeuf博士，负责人）： 突变模型的基因分型 小鼠群体的繁殖策略 组织样本中mRNA的定量PCR 营养干预的膳食准备 骨髓移植手术 通过附属核心间接提供的服务，以动物研究核心作为管道： 糖尿病并发症（微血管、大血管、心脏） 分子生物学（基因测序，核糖探针制备） <$胰岛素分泌/胰岛形态 转基因小鼠的创建 这些服务将由糖尿病内分泌研究中心以收费服务的方式进行 （DERC），新的小鼠代谢表型中心（MMPC），以及生态遗传学中心和 环境卫生（CEEH）。已与这些中心作出安排（见 合作），以便CNRU附属研究者访问上述服务。