CORE--Human Studies

核心——人类研究

• 批准号: 8292228
• 负责人: MICHAEL SCHWARTZ
• 金额: $ 23.57万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8292228
• 关键词: Address; Adipose tissue; Animal Experimentation; Animal Housing; Animals; Area; Arterial Lines; Biomedical Research; Biopsy Specimen; Body Composition; Body Temperature; Bone Marrow Transplantation; Breeding; Budgets; Carbohydrates; Cardiac; Chronic; Clinical Nutrition; Collaborations; Commit; Diabetes Mellitus; Diabetic Angiopathies; Dietary Intervention; Disease; Eating; Educational process of instructing; Endocrinology; Energy Metabolism; Ensure; Environmental Health; Equipment; Euglycemic Clamping; Evolution; Faculty; Fee-for-Service Plans; Floor; Funding; Future; Genes; Genetic; Genotype; Glucose Clamp; Goals; Growth; Health Services Accessibility; Housing; Human; IACUC; Indirect Calorimetry; Interdisciplinary Study; Joints; Laboratories; Letters; Lipids; Measures; Medicine; Messenger RNA; Metabolic; Modeling; Molecular Biology; Monitor; Morphology; Motor Activity; Mus; Nutrient; Nutritional Study; Obesity; Operative Surgical Procedures; Organizational Change; Pattern; Phenotype; Physical activity; Physiology; Preparation; Procedures; Process; Proteins; Protocols documentation; Quality Control; Rattus; Regimen; Research; Research Personnel; Resources; Risk; Rodent; Rodent Model; Schools; Services; Techniques; Testing; Thermogenesis; Time; Tissue Sample; Tissues; Transgenic Mice; Triglycerides; United States National Institutes of Health; Universities; Update; Venous; Washington; Water consumption; base; comparative; energy balance; experience; genetic analysis; implantation; in vivo; insulin secretion; islet; medical schools; meetings; mutant; new technology; nutrition; organizational structure; programs; research facility; response; success

ANIMAL STUDIES CORE I. OVERVIEW OF CHANGES TO THE CORE During the current funding cycle, the Animal Studies Core has been revised with respect both to its organizational structure and to the scope and sophistication of services offered to Affiliate Investigators. These changes have been made possible, in part, through a major increase of institutional commitment in support of the Core's various functions and due to the changing needs of Affiliate Investigators. The following summary highlights these changes: ¿ Organizational changes. Prior to this competing renewal, the CNRU supported basic animal research through two distinct Core laboratories - the Rodent Energy Metabolism and Body Composition Core (Dr. Michael Schwartz, Director) and the Nutrient Gene Core (Dr. Renee LeBoeuf, Director). To better meet the needs of Affiliate Investigators (and in response to input from our External Advisory Board), these two Core laboratories are now merged into a single entity termed the "Animal Studies Core", with Dr. Schwartz serving as Director, Dr. LeBoeuf as Co-Director, and Dr. Gregory Morton as Associate Director. ¿ Institutional commitment. To meet expanding demand for Core services, and in conjunction with the implementation of the new NIH-funded Mouse Metabolic Phenotyping Center (MMPC), the University of Washington School of Medicine has committed new, state-of-the-art research space and animal housing space within its newest biomedical research facility located at the South Lake Union (SLU) campus in downtown Seattle. Institutional funds have also been committed by UW in support of this program, as summarized below: ¿ Research space. Included is an approximate doubling of space for metabolic cage studies, expanding this resource from its current size (8 mouse and 4 rat cages; total of 12 cages) to accommodate 16 cages for rats and 16 for mice (total of 32 cages). New wet lab space will also be made available both for surgical procedures (e.g., implantation of body temperature monitor and chronic indwelling venous and arterial line insertion) and in vivo study procedures (e.g., glucose clamp studies), reflecting an overall marked expansion of research space committed to the Animal Studies Core. (Equipment funds are requested to support this expansion; see Animal Studies Core Budget.) ¿ Adjacent animal housing space. In addition to wet lab space, new animal housing quarters will be installed adjacent to the Animal Studies Core laboratory, allowing study animals received from Affiliate Investigators to be boarded until such time as studies are performed, averting the potential risk to animals located in the regular animal housing area, one floor below the Core laboratory. ¿ Creation of a new UW Obesity/Diabetes Center of Excellence, based at the SLU campus. A key priority of this Center will be to meet current and future animal research needs of investigators involved in obesity research. Additional priorities include support for junior investigators, additional pilot and feasibility funding, and promotion of interdisciplinary research across programs, departments and schools both within and outside of UW. ¿ Transfer of some services to the Analytic Core. During previous funding cycles, the Nutrient Gene Core participated in limited numbers of microassays of proteins, lipids, and carbohydrates, which now has largely been taken over by the Analytic Core. However, staff of the Genetics Component of the Animal Studies Core will continue to teach and advise with respect to quantification of mRNA species, thereby enabling Affiliate Investigators to set up appropriate tests in their own laboratory. Techniques include mRNA isolation from adipose and other tissues. Services offered directly by the Animal Studies Core are: Physiology Component (Dr. Morton, Associate Director): ¿ indirect calorimetry (e.g., metabolic rate, heat production) ¿ locomotor activity/total physical activity ¿ meal pattern analysis/continuous measures of total daily food and water intake ¿ continuous body temperature monitoring ¿ noninvasive body composition analysis ¿ biopsy sample triglyceride content ¿ euglycemic glucose clamp Genetics Component (Dr. LeBoeuf, Director): ¿ genotyping of mutant models ¿ breeding strategy for mouse colonies ¿ quantitative PCR of mRNA in tissue samples ¿ Meal preparation for nutritional interventions ¿ Bone marrow transplantation procedures Services offered indirectly via affiliated cores, with the Animal Studies Core serving as a conduit are: ¿ Diabetes complications (microvascular, macrovascular, cardiac) ¿ Molecular biology (gene sequencing, riboprobe preparation) ¿ Insulin secretion/islet morphology ¿ Creation of transgenic mice These services will be performed on a fee-for-service basis by the Diabetes Endocrinology Research Center (DERC), the new Mouse Metabolic Phenotyping Center (MMPC), and the Center for Ecogenetics and Environmental Health (CEEH). Arrangements have been made with these Centers (see letters of collaboration) for CNRU Affiliate Investigators to have access to the services noted above.

动物研究核心 一、核心的变化概览 在本供资周期内，对动物研究核心进行了修订，其内容包括 组织结构以及向附属调查人员提供的服务的范围和复杂程度。这些 在一定程度上，通过大幅增加机构承诺，以支持以下方面的变化成为可能 核心的各种职能，以及附属调查人员不断变化的需求。以下是摘要 重点介绍了以下更改： ？组织变革。在这次竞争性更新之前，CNRU支持基础动物研究 通过两个不同的核心实验室--啮齿动物能量代谢和身体成分核心(Dr。 Michael Schwartz博士(主任)和营养基因核心(Renee LeBoeuf博士，主任)。为了更好地满足 附属调查人员的需求(并回应我们外部咨询委员会的意见)，这两个核心 实验室现在被合并为一个单独的实体，称为“动物研究核心”，施瓦茨博士 担任主任，勒博夫博士担任联席主任，格雷戈里·莫顿博士担任副主任。 ·机构承诺。以满足不断增长的核心服务需求，并与 美国国立卫生研究院资助的新小鼠代谢表型中心(MMPC)的实施 华盛顿医学院致力于新的、最先进的研究空间和动物住所 其位于南湖联盟(SLU)园区的最新生物医学研究设施内的空间 西雅图市中心。华盛顿大学也承诺提供机构资金来支持这一计划，因为 摘要如下： ？研究空间。包括代谢笼研究的空间大约翻了一番， 将这一资源从目前的规模(8个小鼠笼子和4个大鼠笼子；总共12个笼子)扩展到 容纳16个大鼠笼子和16个小鼠笼子(共32个笼子)。新的潮湿实验室空间也将 可用于外科手术(例如，植入体温监测器 和慢性留置静脉和动脉线插入术)和体内研究程序(例如， 葡萄糖钳研究)，反映了致力于以下方面的研究空间的总体显著扩大 动物研究核心。(需要设备资金来支持这一扩张；见动物 研究核心预算。) ？毗邻的动物住房空间。除了潮湿的实验室空间，新的动物收容所将 安装在动物研究核心实验室附近，允许接收研究动物 从附属调查人员那里登上飞机，直到进行研究时，避免 对位于核心下面一层的常规动物收容所内的动物有潜在危险 实验室。 ？创建一个新的威斯康星州肥胖/糖尿病卓越中心，总部设在SLU校园。一个 该中心的主要优先事项将是满足当前和未来的动物研究需求 参与肥胖研究的调查人员。其他优先事项包括支持初级 调查人员，额外的试点和可行性资金，以及促进跨学科研究 在威斯康星州内外的各个项目、部门和学校。 ？将一些服务转移到Analytic Core。在之前的资金周期中，养分 基因核心参与了有限数量的蛋白质、脂类和 碳水化合物，现在很大程度上已经被分析核心取代。然而，该公司的员工 动物研究核心的遗传学部分将继续教授和提供建议 在量化信使核糖核酸种类方面，从而使附属调查人员能够 在自己的实验室进行适当的测试。技术包括从脂肪中分离出mRNA和 其他纸巾。 动物研究中心直接提供的服务包括： 生理学部分(莫顿博士，副主任)： 间接量热法(如代谢率、产热量) ？运动活动/总体力活动 膳食模式分析/每日食物和水总摄入量的连续测量 ？持续体温监测 ？无创性身体成分分析 活检样本甘油三酯含量 正常血糖葡萄糖钳夹 遗传学部分(LeBoeuf博士，主任)： 突变模型的基因分型 小鼠群体繁育策略 组织标本中mRNA的定量聚合酶链式反应 ？营养干预的膳食准备 ·骨髓移植程序 通过附属核心间接提供的服务，以动物研究核心为渠道： 糖尿病并发症(微血管、大血管、心脏) 分子生物学(基因测序、核探针制备) 胰岛素分泌/胰岛形态 ？转基因小鼠的建立 这些服务将由糖尿病内分泌学研究中心按服务收费提供 (DERC)，新的小鼠代谢表型中心(MMPC)，以及生态遗传学和 环境卫生(CEEH)已与这些中心作出安排(见 合作)，使CNRU附属调查人员能够获得上述服务。