Immune Response to Polyomavirus Infection

对多瘤病毒感染的免疫反应

• 批准号: 7387479
• 负责人: PARMJEET S RANDHAWA
• 金额: $ 38.54万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7387479
• 关键词: Acute; Adoptive Immunotherapy; Allografting; Antibodies; Antigen-Presenting Cells; Antigens; Autoantigens; BK Virus; Binding; Biological Assay; Biological Factors; Biopsy; CD4 Positive T Lymphocytes; Calcineurin inhibitor; Cell physiology; Cells; Clinical; Clinical Course of Disease; Complication; Condition; Cyclosporine; Cyclosporins; Cytotoxic T-Lymphocytes; Data; Development; Diagnosis; Disease; Disease Outcome; Distress; Early Diagnosis; Epitopes; Failure; Frequencies; Funding; Future; Goals; Grant; Helper-Inducer T-Lymphocyte; Human; Human Herpesvirus 4; Humoral Immunities; Hypersensitivity; Immune response; Immune system; Immunity; Immunoglobulin A; Immunoglobulin M; Immunologic Factors; Immunosuppression; Immunosuppressive Agents; Immunotherapy; Incidence; Individual; Infection; Injury; Interferon Type II; Interleukin-10; Interleukin-5; JC Virus; Kidney; Kidney Diseases; Kidney Transplantation; Laboratories; Latent Virus; Lead; Literature; Liver; Lytic Phase; Measurement; Measures; Mediating; Medical center; Memory; Memory Loss; Monitor; Morbidity - disease rate; Mycophenolate Mofetil/Tacrolimus; Organ; Organ Transplantation; Pathogenesis; Patients; Peptides; Perioperative; Peripheral Blood Mononuclear Cell; Pharmaceutical Preparations; Physiologic pulse; Polymerase Chain Reaction; Polyomavirus; Polyomavirus Infections; Production; Proteins; Protocols documentation; Pulse taking; Puncture biopsy; Rate; Recombinants; Reporting; Research; Research Personnel; Risk; Risk Estimate; Serological; Serotyping; Serum; Simian virus 40; Sirolimus; Solid; Subgroup; T Virus; T-Lymphocyte; Testing; Therapeutic immunosuppression; Time; Tissues; Toxic effect; Transplant Recipients; Transplantation; Treatment Protocols; United States National Institutes of Health; Vaccines; Viral; Viral Antigens; Viral Load result; Viral Tumor Antigens; Viremia; Virus Diseases; Virus Latency; antigen binding; base; cell mediated immune response; chemokine; cytokine; cytotoxic; enzyme linked immunospot assay; falls; liver transplantation; lymphoblastoid cell line; outcome forecast; prognostic; programs; response; sialosyl-T antigen; tool; vaccine development

Polyomavirus BK nephropathy is an increasingly recognized complication of organ transplantation. It is associated with significant morbidity & 20-50% risk of graft loss. We propose to study how the immune system reacts to BK virus infection. The ultimate long-term goal of our proposal is to generate data, which will help in the development of vaccines & immunotherapy protocols against this disease. The study will have the following specific aims: (1) To determine the influence of donor and host recipient serologic status on the clinical course of BK virus infection after liver and kidney transplantation. (2) To characterize the anti-BKV T-helper (Th) response in BK virus infection in (a) healthy subjects, (b) kidney transplant recipients, and (c) liver transplant recipients (3) To characterize the T-cytotoxic cell response to BK virus in (a) healthy subjects, (b) kidney transplant recipients, and (c) liver transplant recipients. The information generated during the course of this study will permit earlier diagnosis of BK virus infection infection, prior to advanced and potentially irreversible tissue injury. The research proposed will also promote better understanding of the biologic factors that initiate and perpetuate viral nephropathy. This, in turn, will help us to better predict the clinical course of the disease in individual patients, and help devise more effective forms of anti-viral therapy.

多瘤病毒 BK 肾病是一种日益被认识的器官移植并发症。这是 与显着的发病率和 20-50% 的移植物丢失风险相关。我们建议研究免疫如何 系统对 BK 病毒感染做出反应。我们提案的最终长期目标是生成数据，这 将有助于开发针对这种疾病的疫苗和免疫治疗方案。该研究将有 具体目标如下： (1) 确定供体和宿主受体血清学状态对临床病程的影响 肝肾移植后BK病毒感染的研究进展. (2) 表征 (a) 健康人 BK 病毒感染中的抗 BKV T 辅助细胞 (Th) 反应 受试者，(b) 肾移植受者，和 (c) 肝移植受者 (3) 表征 (a) 健康受试者、(b) 肾脏中 T 细胞毒性细胞对 BK 病毒的反应 移植受者，和 (c) 肝移植受者。 本研究过程中产生的信息将有助于对 BK 病毒感染进行早期诊断 感染，发生在高级且可能不可逆的组织损伤之前。拟议的研究还将促进 更好地了解引发和延续病毒性肾病的生物学因素。反过来，这将 帮助我们更好地预测个体患者疾病的临床病程，并帮助设计出更有效的方案 形式的抗病毒治疗。