AN IN VITRO THREE DIMENSIONAL MODEL SIMULATING PRIMATE EMBRYO IMPLANTATION

模拟灵长类动物胚胎植入的体外三维模型

• 批准号: 7349445
• 负责人: THADDEUS G GOLOS
• 金额: $ 2.72万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7349445
• 关键词: VITRO; THREE; DIMENSIONAL; MODEL; SIMULATING

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. To develop a three-dimensional model with non-human primate embryos to simulate implantation and post- implantation development. Rhesus monkey (macaca mulatta) embryos generated from IVF procedure were used for producing blastocysts. Expanded blastocysts (n= 42) were embedded into a three dimensional environment of Matrigel and cultured in Buffalo rat liver cell-conditioned medium. Hatching of the blastocysts occurred and cell outgrowths and cystic formation were observed beginning at day 5 to 6 post embedding following a rapid development of cellular-like protrusions on the surface of blastocyst implant. In addition, branch-like projections grown into the three- dimensional environment similar to in vivo placental structures were observed. Immunoassay indicated consistent elevations in CG and progesterone secretion. Morphological analysis of embryos in Matrigel explants revealed extensive trophoblast differentiation and migration, degradation of the Matrigel environment by metalloproteinases, and differentiation of non-trophoblast lineages as evidenced by vimentin expresson. This may be a more tractable system for studying early lineage decisions and morphogenesis in the primate embryo. This research used WNPRC Animal Services and Assay Services.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。建立一个非人灵长类动物胚胎的三维模型，以模拟植入和植入后发育。 用体外受精（IVF）方法获得的恒河猴（macacamulatta）胚胎用于生产囊胚。将扩张的囊胚（n= 42）包埋到Matrigel的三维环境中，并在布法罗大鼠肝细胞条件培养基中培养。胚泡孵化发生，并且在胚泡植入物表面上细胞样突起快速发育后，在包埋后第5至6天开始观察到细胞生长和囊性形成。此外，观察到类似于体内胎盘结构的树枝状突起生长到三维环境中。免疫分析表明CG和孕酮分泌的一致升高。在基质胶外植体胚胎的形态学分析揭示了广泛的滋养层分化和迁移，基质胶环境的金属蛋白酶的降解，和非滋养层谱系的分化，证明了波形蛋白expresson。这可能是一个更容易处理的系统，研究早期谱系决策和灵长类动物胚胎的形态发生。本研究使用WNPRC Animal Services和Assay Services。