PROPAGATION OF MONKEY MODELS OF HUMAN DISEASE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。目前对具有特定基因型的动物种群的需求非常大，这不能通过从野外进口动物或通过选择性育种鉴定和繁殖有价值的创始动物来满足。携带主要组织相容性复合体（MHC） 1类等位基因A*01的印度血统恒河猴尤其需要用于疫苗开发研究。本研究的目的是证明辅助生殖技术（ARTs）可以应用于有价值的创始动物的快速、高效繁殖，并利用现有技术生产同卵双胞胎，从而建立一种有效的方法来满足生物医学研究界对动物的需求。专注于纯合子的创始雄性的理由很简单；这只动物产生的所有后代都是Mamu-A*01阳性。此外，如果使用A*01等位基因的杂合子携带者作为卵母细胞供体，则50%的后代将是该等位基因的纯合子，从而产生额外的创始动物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. There is currently a significant need for populations of animals with specified genotypes, which can¿t be satisfied by the importation of animals from the wild, or by the identification and propagation of valuable founder animals by selective breeding. Indian-origin, rhesus macaques carrying the major histocompatability complex (MHC) class 1 allele, A*01, are particularly needed for vaccine development research. The objective of this research is to demonstrate that the assisted reproductive technologies (ARTs) can be applied to the rapid, efficient propagation of a valuable founder animal and to the production of identical twins using existing technology, thereby establishing an effective approach to satisfying animal requirements of the biomedical research community. The rationale for focusing on a homozygous, founder male is simple; all offspring produced by this animal will be Mamu-A*01 positive. Moreover, if the heterozygous carriers of the A*01 allele are used as oocyte donors, 50% of the offspring will be homozygous for the allele, creating additional founder animals.