WHOLE ANIMAL IMAGING TO COMPARE TRANSGENE EXPRESSION IN RODENTS AND PRIMATES

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。目的：在过去的十年中，已经开发了各种技术，可以对活体动物表达的报告基因进行无创成像。核磁共振、PET和光学成像技术都得到了发展，各有优缺点。光学技术之所以成为可能，是因为研制出了灵敏而廉价的CCD相机，也因为人们认识到，从人体内部发出的光传输到外部时，衰减小得惊人。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: In the last decade, a variety of techniques have been developed which allow noninvasive imaging of reporter genes expressed within a live animal. NMR, PET and optical imaging techniques have all been developed and each has its advantages and disadvantages. The optical techniques were made possible by the development of sensitive and inexpensive CCD cameras and also by the realization that light emitted in the interior of the body is transported to the exterior with suprisingly little attentuation.