Imaging: From Super-Resolution to Whole Animal

项目总结（见说明）： 核心C中提供的服务将使该计划项目能够以具有成本效益的方式将急需的组织病理学服务和创新且强大的成像技术纳入该计划。Core C通过提供先进的成像技术来支持计划中的每个项目。超分辨率成像、三维（3D）组织重建、动物活细胞成像 整个动物成像将在下一个申请资助的时期对该计划产生重大影响，实际上已经开始这样做了。Core C正在进行的创新将使这些技术成为用于癌症研究的强大生物工具。重要的是，核心C还通过与世界级病理学家的合作为该计划提供病理支持。 在即将到来的请求支持期间，核心建议通过四个总目标支持计划调查人员。首先，该计划将提供组织学，免疫标记和病理学方面的援助。其次，Core将支持超分辨率成像和活细胞成像，使用全球仅有的20台OMX显微镜之一。第三，核心将有助于在组织水平上成像，无论是使用3D组织重建还是动物活体成像。这些技术都需要定制的显微镜，而这些显微镜通常是不可用的。第四，为项目研究人员提供跟踪肿瘤生长和转移的工具，Core将协助整体动物成像方法，包括生物发光、荧光蛋白和小动物超声。 Core拥有前所未有的成像平台，涵盖超分辨率成像，3D组织重建，组织中的活细胞跟踪和整个动物成像。一个核心，使项目调查人员获得这些技术是必不可少的，因为大多数的成像技术支持的核心是非常复杂的，需要非常专业的培训。此外，获得文书 这些成像技术所需的高成本在没有核心支持的情况下将是令人望而却步的。

PROJECT SUMMARY (See instructions): The services provided in Core C will enable this Program Project to incorporate highly needed histopathology services and innovative and powerful imaging technology into the program in a cost-effective manner. Core C supports every project within the program by providing access to sophisticated imaging technologies. Super-resolution imaging, 3-dimensional (3D) tissue reconstruction, live cell imaging in animals and whole animal imaging will strongly impact the Program during the next period of requested funding, and indeed has already begun to do so. Ongoing innovations by Core C will make these technologies powerful biological tools for the use in cancer research. Importantly, Core C also provides pathological support for the Program through a subcontract with a world-class pathologist. During the upcoming period of requested support, the core proposes to support Program investigators through four general aims. First, the program will provide assistance with histology, immune labeling and pathology. Second, the Core will support super-resolution imaging and live cell imaging using one of only about twenty OMX microscopes that exists woridwide. Third, the core will assist with imaging at the tissue level, either using 3D tissue reconstruction or live imaging in animals. These technologies each require custom built microscopes that are not generally available. Fourth, to provide Program investigators with tools to track tumor growth and metastasis, the Core will assist with whole animal imaging approaches, including bioluminescence, fluorescent proteins and small animal ultrasound. Together, the Core have an unprecedented imaging platform, covering super-resolution imaging, 3D tissue reconstruction, live cell tracking in tissues and whole animal imaging. A core that enables program investigators access to these technologies is essential as most of the imaging technologies supported by the Core are highly sophisticated and require very specialized training. Furthermore, access to the instruments required for these imaging technologies would be cost-prohibitive without the support of a core.