Mechanism interactions and function of the structure specific nuclease XPF

结构特异性核酸酶 XPF 的相互作用机制和功能

• 批准号: BB/D001439/1
• 负责人: Malcolm White
• 金额: $ 28.41万
• 依托单位: University of St Andrews
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2006
• 资助国家: 英国
• 起止时间: 2006 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FD001439%2F1
• 关键词: Mechanism; interactions; function; structure; specific

The archaea are a group of microbes, often found in extreme environments such as volcanic pools and salt pans. With the advent of DNA sequencing, it was recognised that the archaea are not closely related to bacteria such as the ones that make us sick, but rather are more similar to the eukarya (organisms with a nucleus), such as yeast, worms and humans. This relationship is reflected in similarities between the archaea and eukarya in the machinery that makes copies of the genetic material and transfers information (the information processing pathways). For this reason archaea have been studied as a useful model system, but they are also important and interesting in their own right, as they make up a big proportion of the living world. There is still a lot we don't know about information processing in the archaea. We have studied these pathways in archaea for the last 7 years and have discovered and characterised a lot of new proteins. We have been studying the archaeal nuclease XPF (a protein that cuts DNA strands) that is similar to a human protein important for repair of DNA damage. We know the structure of the protein and we wish to carry out studies of its structure, function, mechanism and interactions.

古生菌是一组微生物，通常在极端环境中发现，如火山池和盐田。随着DNA测序的出现，人们认识到古菌与细菌（如使我们生病的细菌）并不密切相关，而是更类似于真核生物（有核生物），如酵母，蠕虫和人类。这种关系反映在古生菌和真核菌在复制遗传物质和传递信息（信息处理途径）的机制上的相似性。由于这个原因，古生菌被作为一个有用的模型系统进行研究，但它们本身也很重要和有趣，因为它们构成了生活世界的很大一部分。关于古细菌的信息处理，我们还有很多不了解的地方。在过去的7年里，我们研究了古细菌中的这些途径，发现并鉴定了许多新蛋白质。我们一直在研究古细菌核酸酶XPF（一种切割DNA链的蛋白质），它与修复DNA损伤的人类蛋白质相似。我们知道蛋白质的结构，我们希望对其结构、功能、机制和相互作用进行研究。

项目成果

Single-molecule characterization of Fen1 and Fen1/PCNA complexes acting on flap substrates.

• DOI: 10.1093/nar/gkt1116
• 发表时间: 2014-02
• 期刊: Nucleic acids research
• 影响因子: 14.9
• 作者: Craggs TD;Hutton RD;Brenlla A;White MF;Penedo JC
• 通讯作者: Penedo JC

PCNA and XPF cooperate to distort DNA substrates.

• DOI: 10.1093/nar/gkp1104
• 发表时间: 2010-03
• 期刊: Nucleic acids research
• 影响因子: 14.9
• 作者: Hutton RD;Craggs TD;White MF;Penedo JC
• 通讯作者: Penedo JC

PCNA stimulates catalysis by structure-specific nucleases using two distinct mechanisms: substrate targeting and catalytic step.

• DOI: 10.1093/nar/gkn745
• 发表时间: 2008-12
• 期刊: NUCLEIC ACIDS RESEARCH
• 影响因子: 14.9
• 作者: Hutton, Richard D.;Roberts, Jennifer A.;Penedo, J. Carlos;White, Malcolm F.
• 通讯作者: White, Malcolm F.