MR MEASUREMENT OF TUMOR PH, PO2 & VASCULARITY IN VIVO & RELATED PROJECTS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。本研究的目的是全面了解与肿瘤生长、发展和治疗反应相关的肿瘤生理学(R01 CA79515-EB002762-05)。特别是，我们关注氧分压和对干预、灌流和跨膜pH的动态反应。氧动力学检测使用FREDOM，这是我们在过去几年开发的一种以六氟苯为报告分子的19F磁共振方法。这使我们能够生成肿瘤氧分压的量化地图，并评估局部对干预的反应。最重要的是，我们现在已经证明，随着干预措施而测量的氧分压变化正确地预测了增强的治疗结果(辐射诱导的生长延迟)。肿瘤的血流灌注通过动态对比增强和BOLD(血氧水平依赖的对比)1HMRI进行评估。相关研究表明，这些参数之间存在密切的相关性，但正如预期的那样，与pO2缺乏相关性。此外，我们正在启动对肿瘤血管的电极、荧光光纤和近红外研究，为解释mri提供相关数据。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this research is to gain a comprehensive understanding of tumor physiology as related to tumor growth, development and response to therapy (R01 CA79515-EB002762-05). In particular, we focus on oxygen tension and the dynamic response to interventions, perfusion and transmembrane pH. Oxygen dynamics are detected using FREDOM, a 19F MRI method using hexafluorobenzene as a reporter molecule, which we have developed over the past several years. This allows us to generate quantitative maps of tumor oxygen tension and to assess local response to interventions. Most significantly, we have now shown that measured changes in oxygen tension accompanying interventions correctly predict enhanced therapeutic outcome (radiation induced growth delay). Tumor perfusion is assessed by dynamic contrast enhancement and BOLD (Blood Oxygen Level Dependant contrast) 1H MRI. Correlative studies have shown a close association between these parameters, but as expected a lack of correlation with pO2. In addition, we are initiating electrode, fluorescent fiber optic and near infrared studies of tumor vasculature providing correlative data to aid in interpreting the MRI