TRACKING OF MELANOSOMES IN MELANOPHORES

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。(i)发现是什么调节了黑素细胞系统中沿微管的细胞器运输：我们将识别与细胞器结合的差异调节蛋白激酶，并确定它们如何调节细胞器运输；（ii）发现色素细胞器沿微管正负端运动之间的协调机制：我们将确定协调是由正负端定向马达与细胞器表面受体蛋白的协同差异结合决定的，还是由随机机制决定的；（iii）发现myosin-V在黑色素细胞和其他细胞类型中的调节机制：我们将利用非洲蟾的黑色素细胞和小鼠巨噬细胞探索间期细胞中myosin-V的调节机制，并确定p21活化激酶（PAK1）在其调节中的作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. (i) find what regulates organelle transport along microtubules in the melanophore system: we will identify differentially regulated protein kinase(s) bound to organelles and determine how they regulate organelle transport; (ii) find the mechanism of coordination between plus and minus-end movement of pigment organelles along microtubules: we will determine if coordination is governed by cooperative differential binding of plus and minus-end directed motors to receptor proteins on the surface of organelles or it is determined by a stochastic mechanism; (iii) find how myosin-V is regulated in melanophores and other cell types: we will explore mechanisms of regulation of myosin-V in interphase cells using Xenopus melanophores and mouse macrophages and determine the role of p21-activated kinase (PAK1) in its regulation.