TRACKING OF MELANOSOMES IN MELANOPHORES

追踪黑色素细胞中的黑色素体

• 批准号: 7357967
• 负责人: Vladimir I Gelfand
• 金额: $ 0.14万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-29 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7357967
• 关键词: microtubules; myosins; organelles

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. (i) find what regulates organelle transport along microtubules in the melanophore system: we will identify differentially regulated protein kinase(s) bound to organelles and determine how they regulate organelle transport; (ii) find the mechanism of coordination between plus and minus-end movement of pigment organelles along microtubules: we will determine if coordination is governed by cooperative differential binding of plus and minus-end directed motors to receptor proteins on the surface of organelles or it is determined by a stochastic mechanism; (iii) find how myosin-V is regulated in melanophores and other cell types: we will explore mechanisms of regulation of myosin-V in interphase cells using Xenopus melanophores and mouse macrophages and determine the role of p21-activated kinase (PAK1) in its regulation.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。(i)找出黑色素细胞系统中细胞器运输沿着微管的调节机制：我们将鉴定与细胞器结合的差异调节蛋白激酶，并确定它们如何调节细胞器运输;（ii）找出色素细胞器沿沿着微管的正端和负端运动之间的协调机制：我们将确定协调是否由合作的正负差异约束来管理，末端定向马达到细胞器表面上的受体蛋白，或者它由随机机制确定;（iii）发现肌球蛋白-V在黑色素细胞和其他细胞类型中是如何调节的：我们将利用非洲爪蟾黑色素细胞和小鼠巨噬细胞探索肌球蛋白-V在间期细胞中的调节机制，并确定p21激活激酶（PAK 1）在其调节中的作用。