Intermediate Filaments: Motility, Motors and Cytoskeletal Interactions
中间丝:运动性、马达和细胞骨架相互作用
基本信息
- 批准号:10227014
- 负责人:
- 金额:$ 25.16万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-06-15 至 2023-07-31
- 项目状态:已结题
- 来源:
- 关键词:AcetylationActinsAdhesionsAffectBeliefBiochemicalBiological ModelsBiotinCRISPR/Cas technologyCell ShapeCellsChemicalsColorCyclic AMP-Dependent Protein KinasesCytoplasmCytoskeletonDataDominant-Negative MutationDynein ATPaseElementsFilamentFocal AdhesionsFundingGenesGeometryGoalsImageIn VitroIndividualIntermediate FilamentsIntracellular TransportInvadedKinesinKnock-outLabelLiteratureMammalian CellMapsMeasuresMechanicsMelanophoresMelanosomesMembraneMesenchymalMicrofilamentsMicrotubulesMitochondriaMolecularMotorMovementOrganellesPhosphorylationPhysiologicalPigmentsPost-Translational Protein ProcessingProteinsProtocols documentationRegulationReporterRoleShapesSignal TransductionSiteStructureSubstrate InteractionSystemTestingTotal Internal Reflection FluorescentTransmembrane TransportValidationVimentincell motilityinhibitor/antagonistlink proteinmembernestin proteintool
项目摘要
Project Summary—Project 2 (Gelfand)
The goal of this proposal is to study dynamic interactions of vimentin intermediate filaments with two other
components of the cytoskeleton, microtubules and actin microfilaments, as well as interactions of VIF with
membrane organelles. We will study how intermediate filaments move along microtubules and identify
microtubule motors that power this movement. We will determine biochemical basis of vimentin interactions
with microtubule motors and identify molecular mechanisms involved in the regulation of these interactions,
such as posttranslational modifications of vimentin. We will also find molecular mechanisms of vimentin
interaction with actin microfilaments and their bundles and with components of focal adhesions. We will test if
interactions of VIF with focal adhesions affect stability of cell-substrate interactions and how vimentin in focal
adhesions modulates cell motility. We will identify molecular linkers between actin and intermediate filaments
and study functional implications of this association. We will also find how intermediate filaments invade focal
adhesions, which focal adhesion proteins link them in place and how intermediate filaments regulate adhesion
turnover. Finally, the role of vimentin filaments in transport of membrane organelles will be investigated using
two model systems, mitochondria and pigment organelles (melanosomes) in melanophores, because both of
these organelles contain bound PKA and PKA-dependent phosphorylation is likely regulated in concert with
intermediate filament dynamics and organelle transport.
项目摘要-项目2(Gelfand)
这个提议的目的是研究波形蛋白中间丝与另外两个中间丝之间的动态相互作用。
细胞骨架,微管和肌动蛋白微丝的组成部分,以及VIF与
膜细胞器我们将研究中间丝如何沿沿着微管移动,
微管发动机来驱动这种运动。我们将确定波形蛋白相互作用的生化基础
与微管马达的相互作用并确定参与这些相互作用调节的分子机制,
例如波形蛋白的翻译后修饰。我们还将发现波形蛋白的分子机制
与肌动蛋白微丝和它们的束的相互作用以及与粘着斑的组分的相互作用。我们将测试如果
VIF与粘着斑的相互作用影响细胞-基质相互作用的稳定性以及VIF粘着斑中波形蛋白的作用
粘附调节细胞运动性。我们将确定肌动蛋白和中间丝之间的分子连接
并研究这种关联的功能含义。我们还将发现中间纤维是如何侵入病灶的
粘附,粘着斑蛋白将它们连接到位,以及中间丝如何调节粘附
周转最后,波形蛋白丝在膜细胞器运输中的作用将通过使用
两个模型系统,线粒体和色素细胞器(黑素体)在黑色素细胞,因为这两个
这些细胞器含有结合的PKA,PKA依赖性磷酸化可能与
中间丝动力学和细胞器运输。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Vladimir I Gelfand其他文献
Fertilization triggers selective degradation of membrane proteins and organelles in C.elegans embryos
受精触发线虫胚胎中膜蛋白和细胞器的选择性降解
- DOI:
- 发表时间:
2011 - 期刊:
- 影响因子:0
- 作者:
Chisako Sakuma;Vladimir I Gelfand;Liqun Luo;Masayuki Miura and Takahiro Chihara;渡辺崇人,三戸太郎,大内淑代,野地澄晴;Ken Naito;佐藤美由紀 - 通讯作者:
佐藤美由紀
NEURONAL MORPHOGENESIS REQUIRES DOGI, A NOVELREGULATOR OF EARLY ENDOSOME ORGANIZATION
神经元形态发生需要 DOGI,一种早期内体组织的新型调节剂
- DOI:
- 发表时间:
2013 - 期刊:
- 影响因子:0
- 作者:
Chisako Sakuma;Vladimir I Gelfand;Liqun Luo;Masayuki Miura and Takahiro Chihara - 通讯作者:
Masayuki Miura and Takahiro Chihara
Vladimir I Gelfand的其他文献
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{{ truncateString('Vladimir I Gelfand', 18)}}的其他基金
Microtubule motors, cytoskeletal organization and cell polarity
微管马达、细胞骨架组织和细胞极性
- 批准号:
10406364 - 财政年份:2019
- 资助金额:
$ 25.16万 - 项目类别:
Microtubule motors, cytoskeletal organization and cell polarity
微管马达、细胞骨架组织和细胞极性
- 批准号:
10626768 - 财政年份:2019
- 资助金额:
$ 25.16万 - 项目类别:
Intermediate Filaments: Motility, Motors and Cytoskeletal Interactions
中间丝:运动性、马达和细胞骨架相互作用
- 批准号:
8142483 - 财政年份:2011
- 资助金额:
$ 25.16万 - 项目类别:
Andor Revolution XD Live Cell Imaging System with Photomanipulation Laser
带光操纵激光的 Andor Revolution XD 活细胞成像系统
- 批准号:
8052665 - 财政年份:2011
- 资助金额:
$ 25.16万 - 项目类别:
Gordon Conference "Motile and Contractile Systems" 2005
戈登会议“运动和收缩系统”2005
- 批准号:
6902468 - 财政年份:2005
- 资助金额:
$ 25.16万 - 项目类别:
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