AN INDUCED-FIT MODEL FOR LANTIBIOTIC AUTOIMMUNITY

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。革兰氏阳性菌乳酸乳杆菌产生乳酸链球菌素，乳酸链球菌素是一种膜孔形成剂，针对其他革兰氏阳性菌的脂质II。为了保护自己免受这种乳酸抗生素的腐蚀性，乳酸乳杆菌产生一种叫做NisI的蛋白质，通过结合乳酸抗生素并使其失活来保护自己免受成孔剂nisin的侵害。我们从以前的研究中知道，NisI在不与其同源底物nisin结合时是无序的。利用光谱偏振法，我们希望表明NisI在与nisin结合后变得有序。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The gram-positive bacterium L. lactis produces the lantibiotic nisin which is a membrane pore forming agent that targets lipid II in other gram-positive organisms. To protect itself from the caustic nature of this lantibiotic, L. lactis produces a protein called NisI which protects itself from the pore-forming agent nisin by binding and inactivating the lantibiotic. We know from previous studies that NisI is disordered when not bound to its cognate substrate nisin. Using spectropolarimetry, we hope to show that NisI becomes ordered upon binding to nisin.