AN INDUCED-FIT MODEL FOR LANTIBIOTIC AUTOIMMUNITY

羊毛硫抗生素自身免疫的诱导拟合模型

• 批准号: 7357987
• 负责人: Satish K Nair
• 金额: $ 0.11万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-29 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7357987
• 关键词: autoimmunity; lipids; model; proteins

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The gram-positive bacterium L. lactis produces the lantibiotic nisin which is a membrane pore forming agent that targets lipid II in other gram-positive organisms. To protect itself from the caustic nature of this lantibiotic, L. lactis produces a protein called NisI which protects itself from the pore-forming agent nisin by binding and inactivating the lantibiotic. We know from previous studies that NisI is disordered when not bound to its cognate substrate nisin. Using spectropolarimetry, we hope to show that NisI becomes ordered upon binding to nisin.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。革兰氏阳性细菌乳酸乳球菌产生羊毛硫抗生素乳链菌肽，这是一种膜孔形成剂，针对其他革兰氏阳性生物中的脂质 II。为了保护自身免受羊毛硫抗生素的腐蚀性影响，乳酸乳球菌会产生一种名为 NisI 的蛋白质，该蛋白质通过结合并灭活羊毛硫抗生素来保护自身免受造孔剂乳链菌肽的侵害。我们从之前的研究中知道，当 NisI 不与其同源底物乳链菌肽结合时，就会发生紊乱。使用分光偏振法，我们希望证明 NisI 在与乳链菌肽结合后变得有序。