Mechanistic Studies of Lantibiotic Biosynthetic and Tailoring Enzymes

羊毛硫抗生素生物合成和定制酶的机理研究

• 批准号: 10647689
• 负责人: Satish K Nair
• 金额: $ 29.15万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10647689
• 关键词: Acids; Address; Anabolism; Anti-Bacterial Agents; Antibiotics; Antiviral Agents; Bacteria; Binding; Biochemical; Biological; Chemistry; Complex; Cross-Linking Reagents; Cryoelectron Microscopy; Cyclization; Data; Dehydration; Electrons; Enzymatic Biochemistry; Enzymes; Glutamic Acid-Specific tRNA; Hand; Hydro-Lyases; Knowledge; Ligase; Lipids; Methodology; Microscopic; Modernization; Modification; Molecular; Multiprotein Complexes; Mutation; N-terminal; Negative Staining; Nisin; Peptides; Phosphotransferases; Post-Translational Protein Processing; Process; Production; Property; Protein Kinase; Proteins; Reaction; Research; Ribonucleoproteins; Ribosomes; Route; Scheme; Structure; Substrate Interaction; Testing; Therapeutic; Transfer RNA; Work; bioactive natural products; catalyst; drug discovery; drug production; functional improvement; lanthionine; peptide natural products; scaffold; small molecule; structural biology; success; synthetic biology; thioether

Project Summary/Abstract Lantibiotics are ribosomally synthesized and post-translationally modified peptide natural products. The biosynthetic enzymes that convert peptide substrates into constrained antibiotics are not well understood, especially with regards to substrate recognition. Here, we seek to carry out biochemical, and structural biological studies on several of these enzymes and address questions regarding substrate recognition, and substrate tolerance. We anticipate that this work will be of use not only towards the discovery of new compounds but to enable the production of peptide-based compounds of any structure.

项目总结/摘要 羊毛硫抗生素是核糖体合成和后修饰肽天然 产品.生物合成酶将肽底物转化为受约束的 对抗生素的了解还不够，特别是关于底物识别。 在这里，我们试图进行生物化学和结构生物学研究的几个 这些酶和解决有关底物识别的问题， 宽容我们预计，这项工作将不仅有助于发现 新的化合物，但能够生产任何肽基化合物， 结构