EXPERIMENTS WITH ANGULAR LIGHT SCATTERING SPECTROSCOPY

角光散射光谱实验

• 批准号: 7357921
• 负责人: MARTIN HUNTER
• 金额: $ 3.05万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7357921
• 关键词: cell nucleus; light scattering; lighting; model; molecular film; particle; polystyrenes; tissues

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have developed a new spectroscopic modality, angular light scattering spectroscopy (ALSS) that enables non-invasive, quantitative assessment of refractive index variations in living cells and tissues, at length scales ranging from several microns (e.g. cell nuclei) to sub-micron features. The technique has been validated on model systems with polydisperse polystyrene beads, and used to characterize both the nuclear and sub-micron particle size distribution in cell monolayer and tissue studies of precancer.

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。我们已经开发了一种新的光谱模式，角光散射光谱（ALSS），它能够非侵入性地定量评估活细胞和组织的折射率变化，长度范围从几微米（例如细胞核）到亚微米特征。该技术已在具有多分散聚苯乙烯珠的模型系统上得到验证，并用于表征细胞单层和癌前组织研究中的核和亚微米粒度分布。