EXPERIMENTS WITH ANGULAR LIGHT SCATTERING SPECTROSCOPY

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。我们已经开发了一种新的光谱模式，角光散射光谱（ALSS），使非侵入性，定量评估的折射率变化在活细胞和组织，在长度范围从几微米（例如细胞核）到亚微米的功能。该技术已在具有多分散聚苯乙烯珠的模型系统上进行了验证，并用于表征癌前病变的细胞单层和组织研究中的核和亚微米粒度分布。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have developed a new spectroscopic modality, angular light scattering spectroscopy (ALSS) that enables non-invasive, quantitative assessment of refractive index variations in living cells and tissues, at length scales ranging from several microns (e.g. cell nuclei) to sub-micron features. The technique has been validated on model systems with polydisperse polystyrene beads, and used to characterize both the nuclear and sub-micron particle size distribution in cell monolayer and tissue studies of precancer.