THEORETICAL ADVANCES OF ANGUALR LIGHT SCATTERING SPECTROSCOPY

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。我们的目标是开发与特征光谱特征（例如，逆幂律散射系数）到特定的亚细胞或组织成分（例如，核染色质、线粒体、表面角蛋白层、胶原基质）。Mie理论和Rayleigh-Gans计算进行了优化的ALSS技术的尺寸选择性。我们还研究了各种分形组织模型对人类上皮细胞的适用性，并通过实验室细胞单层，细胞筏和/或离体组织研究验证了我们的模型预测。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our aim is to develop morphological models correlating characteristic spectral signatures (e.g., inverse power law scattering coefficients) to specific sub-cellular or tissue components (e.g., nuclear chromatin, mitochondria, superficial keratin layer, collagen matrix). Mie theory and Rayleigh-Gans calculations are carried out for optimizing size-selectivity of the ALSS technique. We also examine the applicability of various fractal tissue models to human epithelia, and verify our model predictions via laboratory cell monolayer, cell raft and/or ex vivo tissue studies.