THEORETICAL ADVANCES OF ANGUALR LIGHT SCATTERING SPECTROSCOPY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们的目标是开发将特征光谱特征(例如，逆幂定律散射系数)与特定的亚细胞或组织成分(例如，核染色质、线粒体、表面角蛋白层、胶原基质)相关联的形态模型。用MIE理论和Rayleigh-Gans计算优化了ALSS技术的尺寸选择性。我们还研究和开发了新的人类上皮细胞的分形组织模型，如Born/von Karman模型，并通过实验室细胞单层、细胞筏和/或体外组织研究验证了我们的模型预测。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our aim is to develop morphological models correlating characteristic spectral signatures (e.g., inverse power law scattering coefficients) to specific sub-cellular or tissue components (e.g., nuclear chromatin, mitochondria, superficial keratin layer, collagen matrix). Mie theory and Rayleigh-Gans calculations are carried out for optimizing size-selectivity of the ALSS technique. We also examine and develop new fractal tissue models, such as Born/von Karman model, for human epithelia, and verify our model predictions via laboratory cell monolayer, cell raft and/or ex vivo tissue studies.