MICROBIAL RESPIRATION OF URANIUM & CHROMIUM

铀的微生物呼吸

• 批准号: 7358063
• 负责人: Bradley M. Tebo
• 金额: $ 1.42万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7358063
• 关键词: MICROBIAL; RESPIRATION; URANIUM; CHROMIUM

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this study is to identify the mechanism by which bacteria respire uranium and chromium. For this work, we are focusing on three strains of bacteria, two Shewanella and a Desulfotomaculum, able to respire or reduce both metals. Using advanced microscopic techniques combined with molecular techniques, we hope to identify the genes and proteins involved in the process. TEM, EFTEM and EELS are used to identify the localization and the oxidation state of the metals within the cell which could provide insight into the mechanism involved. Specifically, it will help identify the location of the reduction activity within the cell. The practical application of this work is to improve the knowledge basis for the design of bioremediation strategies. Reduced U and Cr are insoluble and less toxic than their oxidized counterparts. By promoting reduction in a groundwater environment, the toxic metals are immobilized and are less likely to affect drinking or surface water downs

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。这项研究的目的是确定细菌呼吸铀和铬的机制。对于这项工作，我们专注于三种细菌，两种shewanella和一个脱硫菌，能够呼吸或减少两种金属。使用高级微观技术与分子技术结合使用，我们希望鉴定参与过程中涉及的基因和蛋白质。 TEM，EFTEM和EEL用于识别细胞内金属的定位和氧化状态，这些金属可以提供有关所涉及机制的洞察力。具体而言，它将有助于确定细胞内还原活性的位置。这项工作的实际应用是改善设计生物修复策略的知识基础。减少的U和Cr是不溶性的，毒性比其氧化的对应物较小。通过促进地下水环境中的减少，有毒金属被固定了，不太可能影响饮酒或地表水下降