MICROBIAL RESPIRATION OF URANIUM & CHROMIUM

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。这项研究的目的是确定细菌呼吸铀和铬的机制。在这项工作中，我们专注于三种细菌菌株，两种希瓦氏菌和一种脱硫菌，它们能够呼吸或减少这两种金属。利用先进的显微技术和分子技术相结合，我们希望能确定参与这一过程的基因和蛋白质。TEM， EFTEM和EELS用于识别电池内金属的定位和氧化状态，可以深入了解所涉及的机制。具体来说，它将有助于确定细胞内还原活性的位置。本工作的实际应用是提高生物修复策略设计的知识基础。还原后的U和Cr是不溶的，毒性比氧化后的低。通过促进地下水环境的减少，有毒金属被固定，不太可能影响饮用或地表水下降

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this study is to identify the mechanism by which bacteria respire uranium and chromium. For this work, we are focusing on three strains of bacteria, two Shewanella and a Desulfotomaculum, able to respire or reduce both metals. Using advanced microscopic techniques combined with molecular techniques, we hope to identify the genes and proteins involved in the process. TEM, EFTEM and EELS are used to identify the localization and the oxidation state of the metals within the cell which could provide insight into the mechanism involved. Specifically, it will help identify the location of the reduction activity within the cell. The practical application of this work is to improve the knowledge basis for the design of bioremediation strategies. Reduced U and Cr are insoluble and less toxic than their oxidized counterparts. By promoting reduction in a groundwater environment, the toxic metals are immobilized and are less likely to affect drinking or surface water downs