Bacterial genes & proteins involved in redox transform

细菌基因

• 批准号: 6443970
• 负责人: Bradley M. Tebo
• 金额: $ 17.5万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6443970
• 关键词: Bacillus subtilis; Escherichia coli; bacteria; bacterial genetics; bacterial proteins; biotransformation; chromium; copper; environmental toxicology; gene expression; ground water; hazardous substances; manganese; metalloenzyme; metals; oxidation reduction reaction; water pollution

The objective of this project will be to study the mechanisms of transformation and fate of toxic substances primarily in coastal environments. This project will focus on understanding the molecular mechanisms by which metal contaminants are transformed from soluble phases to insoluble phases and to evaluate the contribution from different physiological groups of bacteria in this process. Experiments will center on re-expression of genes and characterization of multi-copper oxidase- like proteins involved in catalyzing Mn (II) oxidation in different bacterial groups. In addition Dr. Tebo has identified several novel bacteria that resist the toxic levels of Cr(VI) and grow with Cr(VI) as an electron acceptor. The second part of his project will examine the biochemical basis for Cr(VI) reduction in these novel Cr(VI)-reducing bacterial and study the regulation of Cr(VI) and the expression of other genes in response to Cr(VI).

该项目的目的是研究主要在沿海环境中的有毒物质的转化和命运机制。本项目将重点了解金属污染物从可溶相转化为不可溶相的分子机制，并评估不同生理菌群在这一过程中的贡献。实验将集中在基因的重新表达和表征多铜氧化酶样蛋白参与催化锰（II）氧化在不同的细菌群。此外，Tebo博士还发现了几种新型细菌，它们可以抵抗Cr（VI）的毒性水平，并以Cr（VI）作为电子受体生长。项目的第二部分将研究这些新型Cr（VI）还原细菌还原Cr（VI）的生化基础，并研究Cr（VI）对Cr（VI）的调控和其他基因对Cr（VI）的表达。